Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Maleimide | - |
Shewanella sp. T3-3 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C600 cells | Shewanella sp. T3-3 |
Protein Variants | Comment | Organism |
---|---|---|
K161S/K184S | the mutant shows much higher residual specific activity after maleimide activation than the wild type enzyme | Shewanella sp. T3-3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
4-nitrophenyl phosphate | at pH 9.8 and 37°C | Shewanella sp. T3-3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Shewanella sp. T3-3 | |
Zn2+ | required | Shewanella sp. T3-3 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Shewanella sp. T3-3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. T3-3 | A0A218PHN9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methoxy-4-(3-phosphatephenyl)spiro [1,2-dioxetane-3,2'-adamantane] disodium salt + H2O | - |
Shewanella sp. T3-3 | ? | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Shewanella sp. T3-3 | 4-nitrophenol + phosphate | - |
? | |
[(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)-methanol 1-(dihydrogenphosphate) disodium salt + H2O | - |
Shewanella sp. T3-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 49500, SDS-PAGE | Shewanella sp. T3-3 |
homodimer | 2 * 45200, calculated from amino acid sequence | Shewanella sp. T3-3 |
Synonyms | Comment | Organism |
---|---|---|
APase | - |
Shewanella sp. T3-3 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 65 | the enzyme remains stable after 24 h incubation between 20 and 65°C | Shewanella sp. T3-3 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7000 | - |
4-nitrophenyl phosphate | at pH 9.8 and 37°C | Shewanella sp. T3-3 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | the enzyme remains stable after 24 h incubation between pH 6.0 and 11.0 | Shewanella sp. T3-3 |