Literature summary for 3.1.3.1 extracted from

Tang, J.B.; Yang, H.M.; Liang, S.J.; Chen, Y.; Mu, Q.J.; Zhang, J.B.
Comparative characterization of recombinant ZZ protein-alkaline phosphatase and its application in enzyme immunoassays (2013), Appl. Microbiol. Biotechnol., 97, 153-158.

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Application

Application	Comment	Organism
analysis	construction o a fusion proein between the ZZ protein, two specific Fc-fragment-binding domains derived from the B-domain of SpA, and alkaline phosphatase. The ZZ-AP fusion retains full parental activities and exhibits an approximately tenfold higher sensitivity than that of ZZ-AP conjugate in enzyme-linked immunosorbent assay. The ZZ-AP fusion is a promising immunoreagent for IgG detection and a potential biolinker between antibodies and reporter enzymes. Compared with the parents, the equilibrium dissociation constant of ZZ-AP conjugate is decreased by 32% and catalytic activity is decreased by 24%	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	4-nitrophenyl phosphate	fusion protein with ZZ protein, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
0.29	-	4-nitrophenyl phosphate	native enzyme, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
0.31	-	4-nitrophenyl phosphate	conjugate between enzyme and ZZ protein, pH not specified in the publication, temperature not specified in the publication	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Escherichia coli	4-nitrophenol + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
285	-	4-nitrophenyl phosphate	conjugate between enzyme and ZZ protein, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
370	-	4-nitrophenyl phosphate	fusion protein with ZZ protein, pH not specified in the publication, temperature not specified in the publication	Escherichia coli
378	-	4-nitrophenyl phosphate	native enzyme, pH not specified in the publication, temperature not specified in the publication	Escherichia coli

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Release 2023.1 (January 2023)