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Literature summary for 3.1.3.1 extracted from

  • Dziedziejko, V.; Safranow, K.; Slowik-Zylka, D.; Machoy-Mokrzynska, A.; Millo, B.; Machoy, Z.; Chlubek, D.
    Characterisation of rat and human tissue alkaline phosphatase isoforms by high-performance liquid chromatography and agarose gel electrophoresis (2009), Biochimie, 91, 445-452.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
L-phenylalanine 10 mM, 100% inhibition; 10 mM, 100% inhibition; 10 mM, 37% inhibition; 10 mM, 37% inhibition, bone isoenzyme; 10 mM, 62% inhibition, isoenzyme rTI2A; 10 mM, 70% inhibition, isoenzyme rTI2B Rattus norvegicus
lectin 5 g/L, precipitation by lectin: 100%; 5 g/L, precipitation by lectin: 100%; 5 g/L, precipitation by lectin: 100%, bone isoenzyme; 5 g/L, precipitation by lectin: 58%, isoenzyme rTI2B; 5 g/L, precipitation by lectin: 76%, isoenzyme rTI2A Rattus norvegicus
Urea 3 mM, 100% inhibition; 3 mM, 100% inhibition; 3 mM, 68% inhibition, bone isoenzyme; 3 mM, 70% inhibition, isoenzyme rTI2B; 3 mM, 71% inhibition; 3 mM, 80% inhibition, isoenzyme rTI2A Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane ALP is found in the soluble form as well as in the insoluble form bound to the mebrane via a GPI anchor Rattus norvegicus 16020
-
soluble ALP is found in the soluble form as well as in the insoluble form bound to the mebrane via a GPI anchor Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus P08289
-
-

Purification (Commentary)

Purification (Comment) Organism
by using HPLC separation of a intestinal homogenate two ALP peaks (rTI2A and rTI2B) are obtained Rattus norvegicus
by using HPLC separation of a rat bone homogenate one peak is obtained Rattus norvegicus
by using HPLC separation of a rat liver homogenate three peaks are obtained (rTL1, rTL2 and rTL3) Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
bone
-
Rattus norvegicus
-
intestine
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
56% of intestinal activity is retained using HPLC for separation Rattus norvegicus
additional information
-
57% of intestinal activity is retained using HPLC for separation. Increased activity is detected after cleaving ALP from the cellular membrane by GPI-specific phospholipase C Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Rattus norvegicus 4-nitrophenol + phosphate
-
?

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Rattus norvegicus
ALP liver isoform Rattus norvegicus
ALP bone isoform Rattus norvegicus
ALP intestinal isoform Rattus norvegicus
rTB
-
Rattus norvegicus
rTI2A
-
Rattus norvegicus
rTI2B
-
Rattus norvegicus
rTL1
-
Rattus norvegicus
rTL2
-
Rattus norvegicus
rTL3
-
Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
15 min, 100% inactivation Rattus norvegicus
56
-
15 min, 27% inactivation, isoenzyme rTI2B Rattus norvegicus
56
-
15 min, 32% inactivation Rattus norvegicus
56
-
15 min, 52% inactivation, isoenzyme rTI2A Rattus norvegicus
56
-
15 min, 78% inactivation, bone isoenzyme Rattus norvegicus