Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-phenylalanine | 10 mM, 100% inhibition; 10 mM, 100% inhibition; 10 mM, 37% inhibition; 10 mM, 37% inhibition, bone isoenzyme; 10 mM, 62% inhibition, isoenzyme rTI2A; 10 mM, 70% inhibition, isoenzyme rTI2B | Rattus norvegicus | |
lectin | 5 g/L, precipitation by lectin: 100%; 5 g/L, precipitation by lectin: 100%; 5 g/L, precipitation by lectin: 100%, bone isoenzyme; 5 g/L, precipitation by lectin: 58%, isoenzyme rTI2B; 5 g/L, precipitation by lectin: 76%, isoenzyme rTI2A | Rattus norvegicus | |
Urea | 3 mM, 100% inhibition; 3 mM, 100% inhibition; 3 mM, 68% inhibition, bone isoenzyme; 3 mM, 70% inhibition, isoenzyme rTI2B; 3 mM, 71% inhibition; 3 mM, 80% inhibition, isoenzyme rTI2A | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | ALP is found in the soluble form as well as in the insoluble form bound to the mebrane via a GPI anchor | Rattus norvegicus | 16020 | - |
soluble | ALP is found in the soluble form as well as in the insoluble form bound to the mebrane via a GPI anchor | Rattus norvegicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | P08289 | - |
- |
Purification (Comment) | Organism |
---|---|
by using HPLC separation of a intestinal homogenate two ALP peaks (rTI2A and rTI2B) are obtained | Rattus norvegicus |
by using HPLC separation of a rat bone homogenate one peak is obtained | Rattus norvegicus |
by using HPLC separation of a rat liver homogenate three peaks are obtained (rTL1, rTL2 and rTL3) | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone | - |
Rattus norvegicus | - |
intestine | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
56% of intestinal activity is retained using HPLC for separation | Rattus norvegicus |
additional information | - |
57% of intestinal activity is retained using HPLC for separation. Increased activity is detected after cleaving ALP from the cellular membrane by GPI-specific phospholipase C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Rattus norvegicus |
ALP | liver isoform | Rattus norvegicus |
ALP | bone isoform | Rattus norvegicus |
ALP | intestinal isoform | Rattus norvegicus |
rTB | - |
Rattus norvegicus |
rTI2A | - |
Rattus norvegicus |
rTI2B | - |
Rattus norvegicus |
rTL1 | - |
Rattus norvegicus |
rTL2 | - |
Rattus norvegicus |
rTL3 | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
15 min, 100% inactivation | Rattus norvegicus |
56 | - |
15 min, 27% inactivation, isoenzyme rTI2B | Rattus norvegicus |
56 | - |
15 min, 32% inactivation | Rattus norvegicus |
56 | - |
15 min, 52% inactivation, isoenzyme rTI2A | Rattus norvegicus |
56 | - |
15 min, 78% inactivation, bone isoenzyme | Rattus norvegicus |