Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli and purified as a hexa-His-tagged fusion protein | Geobacillus thermodenitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, 99% inhibition | Geobacillus thermodenitrificans | |
inorganic phosphate | - |
Geobacillus thermodenitrificans | |
additional information | enzyme is resistant to urea and dithioreitol | Geobacillus thermodenitrificans | |
vanadate | 10 mM, 89% inhibition | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0315 | - |
4-nitrophenyl phosphate | - |
Geobacillus thermodenitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | a divalent cation is essential, with a combination of Mg2+ and Co2+ or Zn2+ preferred | Geobacillus thermodenitrificans | |
Mg2+ | a divalent cation is essential, with a combination of Mg2+ and Co2+ or Zn2+ preferred | Geobacillus thermodenitrificans | |
Zn2+ | a divalent cation is essential, with a combination of Mg2+ and Co2+ or Zn2+ preferred | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42150 | - |
deduced from cDNA | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A8WEG4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Geobacillus thermodenitrificans | 4-nitrophenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Geobacillus thermodenitrificans |
Gtd AP | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
optimal temperature | Geobacillus thermodenitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | enzyme retains a high activity between 45°C-60°C | Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme gets quickly inactivated at 80°C for 15 min, exhibiting a half-life of 8 min at 70°C | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Geobacillus thermodenitrificans |