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Literature summary for 3.1.3.1 extracted from

  • So, P.P.; Tsui, F.W.; Vieth, R.; Tupy, J.H.; Pritzker, K.P.
    Inhibition of alkaline phosphatase by cysteine: implications for calcium pyrophosphate dihydrate crystal deposition disease (2007), J. Rheumatol., 34, 1313-1322.
    View publication on PubMed

Application

Application Comment Organism
medicine the control of endogenous inhibitors, such as cysteine, that interfere with the ability of tissue nonspecific alkaline phosphatase to regulate calcium pyrophosphate dihydrate crystal formation and dissolution in joints could be a potential therapeutic option for calcium pyrophosphate dihydrate crystal deposition disease Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cys uncompetitive inhibition of phosphatase activity, mixed-competitive inhibition of inorganic pyrophosphatase activity. Inorganic pyrophosphatase activity is inhibited more than phosphatase activity. Ca2+ and Mg2+ ion concentrations may regulate this inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0156
-
4-nitrophenyl phosphate 37°C, pH 7.4, 1 mM Ca2+ Homo sapiens
0.019
-
4-nitrophenyl phosphate 37°C, pH 7.4, with Ca2+ and Mg2+ Homo sapiens
0.027
-
4-nitrophenyl phosphate 37°C, pH 7.4, 1 mM Ca2+, 1 mM Mg2+ Homo sapiens
0.035
-
4-nitrophenyl phosphate 37°C, pH 7.4, 1 mM Mg2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
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Synonyms

Synonyms Comment Organism
tissue nonspecific alkaline phosphatase
-
Homo sapiens
TNAP
-
Homo sapiens