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Literature summary for 3.1.3.1 extracted from

  • Guimaraes, L.H.; Junior, A.B.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.L.
    Purification and biochemical characterization of a mycelial alkaline phosphatase without DNAase activity produced by Aspergillus caespitosus (2007), Folia Microbiol. (Praha), 52, 231-236.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4Cl 10 mM, activity is enhanced to 165% of control Aspergillus caespitosus

Application

Application Comment Organism
molecular biology the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity, indicating its potential for recombinant DNA technology Aspergillus caespitosus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 1 mM, 32% inhibition Aspergillus caespitosus
CuCl2 10 mM, 24% inhibition Aspergillus caespitosus
CuSO4 10 mM, 71% inhibition Aspergillus caespitosus
EDTA 10 mM, 71% inhibition Aspergillus caespitosus
HgCl2 10 mM, 74%inhibition Aspergillus caespitosus
ZnCl2 10 mM, 58% inhibition Aspergillus caespitosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
4-nitrophenyl phosphate without Mg2+ Aspergillus caespitosus
0.41
-
4-nitrophenyl phosphate with Mg2+ Aspergillus caespitosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM MgSO4, activity is enhanced to 239% of control Aspergillus caespitosus
MnCl2 10 mM, activity is enhanced to 138% of control Aspergillus caespitosus
NaCl 10 mM, activity is enhanced to 151% of control Aspergillus caespitosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
2 * 71000, SDS-PAGE Aspergillus caespitosus
138000
-
gel filtration Aspergillus caespitosus

Organism

Organism UniProt Comment Textmining
Aspergillus caespitosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 74% saccharide content Aspergillus caespitosus

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus caespitosus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus caespitosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50.4
-
-
Aspergillus caespitosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phosphoglycerate + H2O 53.7% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus glycerol + phosphate
-
?
3'-ITP + H2O 51.5% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
?
4-nitrophenyl phosphate + H2O
-
Aspergillus caespitosus 4-nitrophenol + phosphate
-
?
adenosine 5'-(beta-glucosyl)diphosphate + H2O 29.8% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
?
ATP + H2O 57.8% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
?
D-fructose 6-phosphate + H2O 5.2% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus D-fructose + phosphate
-
?
D-glucose 6-phosphate + H2O 19.4% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus D-glucose + phosphate
-
?
glucose 1-phosphate + H2O 93% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus glucose + phosphate
-
?
additional information the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity Aspergillus caespitosus ?
-
?
phosphoserine + H2O 9.3% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus phosphate + Ser
-
?
phosphothreonine + H2O 22.1% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus phosphate + Thr
-
?
phosphotyrosine + H2O 23.7% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus phosphate + Tyr
-
?
UTP + H2O 67% of the activity with 4-nitrophenyl phosphate Aspergillus caespitosus ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 71000, SDS-PAGE Aspergillus caespitosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Aspergillus caespitosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 85 65°C: about 55% of maximal activity, 85°C: about 55% maximal activity Aspergillus caespitosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
60 min, about 5% of maximal activity Aspergillus caespitosus
70
-
60 min, about 50% loss of activity Aspergillus caespitosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Aspergillus caespitosus

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 pH 8.0: about 65% of maximal activity, pH 10.5: about 95% of maximal activity Aspergillus caespitosus