Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4Cl | 10 mM, activity is enhanced to 165% of control | Aspergillus caespitosus |
Application | Comment | Organism |
---|---|---|
molecular biology | the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity, indicating its potential for recombinant DNA technology | Aspergillus caespitosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, 32% inhibition | Aspergillus caespitosus | |
CuCl2 | 10 mM, 24% inhibition | Aspergillus caespitosus | |
CuSO4 | 10 mM, 71% inhibition | Aspergillus caespitosus | |
EDTA | 10 mM, 71% inhibition | Aspergillus caespitosus | |
HgCl2 | 10 mM, 74%inhibition | Aspergillus caespitosus | |
ZnCl2 | 10 mM, 58% inhibition | Aspergillus caespitosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
4-nitrophenyl phosphate | without Mg2+ | Aspergillus caespitosus | |
0.41 | - |
4-nitrophenyl phosphate | with Mg2+ | Aspergillus caespitosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM MgSO4, activity is enhanced to 239% of control | Aspergillus caespitosus | |
MnCl2 | 10 mM, activity is enhanced to 138% of control | Aspergillus caespitosus | |
NaCl | 10 mM, activity is enhanced to 151% of control | Aspergillus caespitosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
2 * 71000, SDS-PAGE | Aspergillus caespitosus |
138000 | - |
gel filtration | Aspergillus caespitosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus caespitosus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 74% saccharide content | Aspergillus caespitosus |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus caespitosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus caespitosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50.4 | - |
- |
Aspergillus caespitosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phosphoglycerate + H2O | 53.7% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | glycerol + phosphate | - |
? | |
3'-ITP + H2O | 51.5% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | ? | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Aspergillus caespitosus | 4-nitrophenol + phosphate | - |
? | |
adenosine 5'-(beta-glucosyl)diphosphate + H2O | 29.8% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | ? | - |
? | |
ATP + H2O | 57.8% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | ? | - |
? | |
D-fructose 6-phosphate + H2O | 5.2% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | D-fructose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 19.4% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | D-glucose + phosphate | - |
? | |
glucose 1-phosphate + H2O | 93% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | glucose + phosphate | - |
? | |
additional information | the purified alkaline phosphatase removes the 5'-phosphate group of a linearized plasmid without showing DNAase activity | Aspergillus caespitosus | ? | - |
? | |
phosphoserine + H2O | 9.3% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | phosphate + Ser | - |
? | |
phosphothreonine + H2O | 22.1% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | phosphate + Thr | - |
? | |
phosphotyrosine + H2O | 23.7% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | phosphate + Tyr | - |
? | |
UTP + H2O | 67% of the activity with 4-nitrophenyl phosphate | Aspergillus caespitosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 71000, SDS-PAGE | Aspergillus caespitosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Aspergillus caespitosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | 65°C: about 55% of maximal activity, 85°C: about 55% maximal activity | Aspergillus caespitosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, about 5% of maximal activity | Aspergillus caespitosus |
70 | - |
60 min, about 50% loss of activity | Aspergillus caespitosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Aspergillus caespitosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10.5 | pH 8.0: about 65% of maximal activity, pH 10.5: about 95% of maximal activity | Aspergillus caespitosus |