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Literature summary for 3.1.3.1 extracted from
- Structural and kinetic alterations of constitutive conidial alkaline phosphatase from the osmotically-sensitive mutant of Neurospora crassa (2006), Folia Microbiol. (Praha), 51, 431-437.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Neurospora crassa |  |
| vanadate | - |
Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
4-nitrophenyl phosphate | 37°C, pH 9.5, mutant os-1 enzyme, without MgCl2 | Neurospora crassa | |
| 0.27 | - |
4-nitrophenyl phosphate | 37°C, pH 9.5, wild-type enzyme, without MgCl2 | Neurospora crassa | |
| 0.4 | - |
4-nitrophenyl phosphate | 37°C, pH 9.5, mutant os-1 enzyme, 2 mM MgCl2 | Neurospora crassa | |
| 1.2 | - |
4-nitrophenyl phosphate | 37°C, pH 9.5, wild-type enzyme, 2 mM MgCl2 | Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 26% stimulation of hydrolysis of 4-nitrophenyl phosphate. KM: 0.0022 mM for wild-type enzyme, 0.00051 mM for mutant os-1 enzyme | Neurospora crassa | |
| Mg2+ | 80% stimulation of hydrolysis of 4-nitrophenyl phosphate. KM: 0.0036 mM for wild-type enzyme, 0.0084 mM for mutant os-1 enzyme | Neurospora crassa | |
| Ni2+ | 23% stimulation of hydrolysis of 4-nitrophenyl phosphate. KM: 0.0019 mM for wild-type enzyme, 0.0037 mM for mutant os-1 enzyme | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000 + 1 * 62000, SDS-PAGE | Neurospora crassa |
| 62000 | - |
2 * 36000 + 1 * 62000, SDS-PAGE | Neurospora crassa |
| 137000 | - |
- |
Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
wild-type strain osmotically-sensitive mutant os-1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 16% saccharide | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| conidium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 173.4 | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 5 mM Tris-HCl buffer, stable for up to 6 months | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phosphoglycerate + H2O | wild-type activity is 16.9% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 16.3fold higher than activity from wild-type enzyme | Neurospora crassa | glycerol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | activity with mutant os-1 enzyme is 12.2fold higher than activity from wild-type enzyme | Neurospora crassa | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | wild-type activity is 16.9% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 1.2fold lower than activity from wild-type enzyme | Neurospora crassa | AMP + phosphate | - |
? | |
| AMP + H2O | wild-type activity is 9.6% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 18.2fold higher than activity from wild-type enzyme | Neurospora crassa | adenosine + phosphate | - |
? | |
| ATP + H2O | wild-type activity is 15.4% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 6.9fold higher than activity from wild-type enzyme | Neurospora crassa | ? | - |
? | |
| D-fructose 6-phosphate + H2O | wild-type activity is 2.2% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 52fold higher than activity from wild-type enzyme | Neurospora crassa | D-fructose + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | wild-type activity is 9.6% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 21fold higher than activity from wild-type enzyme | Neurospora crassa | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | wild-type activity is 6.6% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 34.5fold higher than activity from wild-type enzyme | Neurospora crassa | D-glucose + phosphate | - |
? | |
| additional information | no hydrolysis of diphosphate | Neurospora crassa | ? | - |
? | |
| O-phosphoserine + H2O | wild-type activity is 14.0% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 10.9fold higher than activity from wild-type enzyme | Neurospora crassa | serine + phosphate | - |
? | |
| O-phosphothreonine + H2O | wild-type activity is 21.3% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 9.8fold higher than activity from wild-type enzyme | Neurospora crassa | threonine + phosphate | - |
? | |
| O-phosphotyrosine + H2O | wild-type activity is 68.3% of wild-type activity with 4-nitrophenyl phosphate. Activity with mutant os-1 enzyme is 8.6fold higher than activity from wild-type enzyme | Neurospora crassa | tyrosine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 2 * 36000 + 1 * 62000, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type strain | Neurospora crassa |
| 65 | - |
os-1 mutant | Neurospora crassa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | 30°C: about 60% of maximal activity of wild-type enzyme, about 45% of maximal activity of mutant enzyme os-1, 75°C: about 40% of maximal activity of wild-type enzyme, about 70% of maximal activity of mutant enzyme os-1 | Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
stable for 10 days | Neurospora crassa |
| 60 | - |
biphasic thermoinactivation, t1/2 of mutant enzyme os-1 is 31.7 min for the fast phase and 3.0 h for the slow phase. The wild-type enzyme shows t1/2 values of 4.8 min and 80.3 min, respectively | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | pH 8.0: about 50% of maximal activity of mutant enzyme os-1, pH 10: about 95% of maximal activity of mutant enzyme os-1 | Neurospora crassa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00056 | - |
vanadate | 37°C, pH 9.5, wild-type enzyme | Neurospora crassa | |
| 0.0075 | - |
vanadate | 37°C, pH 9.5, mutant os-1 enzyme | Neurospora crassa | |
| 0.55 | - |
phosphate | 37°C, pH 9.5, mutant os-1 enzyme | Neurospora crassa | |
| 1.6 | - |
phosphate | 37°C, pH 9.5, wild-type enzyme | Neurospora crassa | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Neurospora crassa | isoelectric focusing | - |
2.7 |
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