Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 90% inhibition at 1 mM and 25°C, complete inhibition at 0.1 mM EDTA and 90°C | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
4-nitrophenyl phosphate | pH 8.0, 65°C | Thermotoga maritima | |
0.175 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metalloenzyme, dependent on, can partially be substituted by Mg2+ and Mn2+ | Thermotoga maritima | |
Mg2+ | metalloenzyme, can partially substitute for Co2+ | Thermotoga maritima | |
Mn2+ | metalloenzyme, can partially substitute for Co2+ | Thermotoga maritima | |
additional information | effect of metal ions, overview | Thermotoga maritima | |
Zn2+ | metalloenzyme, 0.3 mol zinc per mol of monomer, binding structure | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
8 * 45000, sucrose-density gradient sedimentation, quarternary structure, conformation of the majority of the enzyme | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, substrate binding and active site structure | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Thermotoga maritima | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 45000, sucrose-density gradient sedimentation, quarternary structure, conformation of the majority of the enzyme | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Thermotoga maritima |
AP | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable for more than 24 h | Thermotoga maritima |
90 | - |
stable for 5 h | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
4-nitrophenyl phosphate | pH 8.0, 25°C | Thermotoga maritima | |
100 | - |
4-nitrophenyl phosphate | pH 8.0, 65°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermotoga maritima |