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Literature summary for 3.1.3.1 extracted from

  • Yang, D.; Wang, J.; Bao, Y.; An, L.
    Purification and characterization of Ulva pertusa Kjellm alkaline phosphatase (2003), Prep. Biochem. Biotechnol., 33, 113-123.
    View publication on PubMed
Search for more literature for 3.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
 Ulva pertusa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.95
-
 4-nitrophenyl phosphate pH 9.8, 37°C Ulva pertusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19500
-
 x * 19500, SDS-PAGE Ulva pertusa

Organism

Organism UniProt Comment Textmining
Ulva pertusa
-
 Kjelm
-
Ulva pertusa Kjelm
-
 Kjelm
-

Purification (Commentary)

Purification (Comment) Organism
1230fold, to homogeneity Ulva pertusa

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Ulva pertusa
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.6
-
 purified enzyme Ulva pertusa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Ulva pertusa 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Ulva pertusa Kjelm 4-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 19500, SDS-PAGE Ulva pertusa

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
 Ulva pertusa
ALP
-
 Ulva pertusa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Ulva pertusa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
-
 Ulva pertusa