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Literature summary for 3.1.3.1 extracted from

  • Le Du, M.H.; Stigbrand, T.; Taussig, M.J.; Menez, A.; Stura, E.A.
    Crystal structure of alkaline phosphatase from human placenta at 1.8 A resolution. Implication for a substrate specificity (2001), J. Biol. Chem., 276, 9158-9165.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
10-16 mg/ml purified enzyme in 10 mM Tris, pH 7.0, 2 mM MgCl2, 0.02% NaN3, sitting drop vapour diffusion method, free or in complex with substrate 4-nitrophenyl phosphate, reservoir solution: 12-14% PEG 4000, 20% PEG 3350, 2 mM zinc acetate, 100 mM sodium cacodylate, pH 6.5, the buffer can also be acetate, citrate or imidazole malate, X-ray diffraction structure determination and analysis at 1.9 A resolution Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-amino acids specific uncompetitive inhibition, molecular mechanism involves Arg166 and Glu429 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ metalloenzyme, metal binding site structure Homo sapiens
Zn2+ metalloenzyme, metal binding site structure Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P05187 humanplacenta alkaline phosphatase PLAP
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Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein gylcosylation site Asn249 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
from placenta Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, active site structure, highly conserved in evolution of alkaline phosphatases Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta tissue-specific enzyme Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O allosteric mechanism Homo sapiens 4-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
dimer crystal structure Homo sapiens

Synonyms

Synonyms Comment Organism
alkaline phosphatase
-
Homo sapiens
AP
-
Homo sapiens
PLAP
-
Homo sapiens