Literature summary for 3.1.27.8 extracted from

Morita, Y.; Shibutani, T.; Nakanishi, N.; Nishikura, K.; Iwai, S.; Kuraoka, I.
Human endonuclease V is a ribonuclease specific for inosine-containing RNA (2013), Nat. Commun., 4, 2273.

Search for more literature for 3.1.27.8

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of C-terminally FLAG-V5-His6-tagged enzyme in HEK293 cell, recombinant expression of N-terminally GST-tagged wild-type and mutant enzymes in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D52A	site-directed mutagenesis of an active site residue, inactive mutant	Homo sapiens
E100A	site-directed mutagenesis of an active site residue, the mutant shows highly reduced activity compared to the wild-type enzyme	Homo sapiens
Y91A	site-directed mutagenesis of an active site residue, the mutant shows highly reduced activity compared to the wild-type enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, recombinant N-terminally GST-tagged wild-type and mutant enzymes, SDS-PAGE	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	the human endonuclease V ribonuclease is specific for inosine-containing RNA, it preferentially binds to RNA and efficiently hydrolyses the second phosphodiester bond located 3' to the inosine in unpaired inosine-containing ssRNA regions in dsRNA, at 5'-IIUI-3' and 5'-UIUU-3'. But hEndoV can also cleave ssDNA substrates containing deoxyinosine. RNA is preferred to DNA by the enzyme as a substrate	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q8N8Q3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally GST-tagged wild-type and mutant enzymes from Escherichia coli by glutathione affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
fibroblast	dermal	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the human endonuclease V ribonuclease is specific for inosine-containing RNA, it preferentially binds to RNA and efficiently hydrolyses the second phosphodiester bond located 3' to the inosine in unpaired inosine-containing ssRNA regions in dsRNA, at 5'-IIUI-3' and 5'-UIUU-3'. But hEndoV can also cleave ssDNA substrates containing deoxyinosine. RNA is preferred to DNA by the enzyme as a substrate	Homo sapiens	?	-	?
additional information	32P-labelled 30mer ssDNA containing deoxyinosine and/or 21mer ssRNA containing inosine are used as substrates, as well as 32P-labelled 30mer ssDNA containing deoxyinosine	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 55000, recombinant N-terminally GST-tagged wild-type and mutant enzymes, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
endonuclease V	-	Homo sapiens
FLJ35220	-	Homo sapiens
FLJ35220 protein	-	Homo sapiens
hEndoV	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)