BRENDA - Enzyme Database show
show all sequences of 3.1.27.10

The action mode of the ribosome-inactivating protein alpha-sarcin

Hwu, L.; Huang, K.; Chen, D.; Lin, A.; J. Biomed. Sci. 7, 420-428 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template
Aspergillus giganteus
Engineering
Amino acid exchange
Commentary
Organism
C148S
point mutation constructed from cDNA clone
Aspergillus giganteus
C6S
point mutation constructed from cDNA clone
Aspergillus giganteus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
rRNA + H2O
Aspergillus giganteus
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus giganteus
-
filamentous fungus, mold
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Aspergillus giganteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
RNA + H2O
hydrolyzes naked RNA at purines in both single-and double-stranded regions
646312
Aspergillus giganteus
?
-
-
-
?
rRNA + H2O
-
646312
Aspergillus giganteus
?
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template
Aspergillus giganteus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C148S
point mutation constructed from cDNA clone
Aspergillus giganteus
C6S
point mutation constructed from cDNA clone
Aspergillus giganteus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
rRNA + H2O
Aspergillus giganteus
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Aspergillus giganteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
RNA + H2O
hydrolyzes naked RNA at purines in both single-and double-stranded regions
646312
Aspergillus giganteus
?
-
-
-
?
rRNA + H2O
-
646312
Aspergillus giganteus
?
-
-
-
?
Other publictions for EC 3.1.27.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730369
Alford
Conditional protein splicing o ...
Aspergillus giganteus
Mol. Biosyst.
10
831-837
2014
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730862
Carreras-Sangra
Production and characterizatio ...
Aspergillus giganteus, Aspergillus giganteus MDH18894
Protein Eng. Des. Sel.
25
425-435
2012
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730992
He
Mechanisms for ribotoxin-induc ...
Aspergillus giganteus
Toxicol. Appl. Pharmacol.
265
10-18
2012
2
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2
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710149
Garcia-Ortega
Cleavage of the sarcin-ricin l ...
Aspergillus giganteus
Nucleic Acids Res.
38
4108-4119
2010
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690631
Alvarez-Garcia
Role of the basic character of ...
Fungi
Arch. Biochem. Biophys.
481
37-44
2009
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707861
Alford
alpha-Sarcin catalytic activit ...
Aspergillus giganteus
BMC Biochem.
10
9-9
2009
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683339
Jandhyala
ZAK: a MAP3Kinase that transdu ...
Escherichia coli
Cell. Microbiol.
10
1468-1477
2008
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704754
Alvarez-Garcia
Lactococcus lactis as a vehicl ...
Lactococcus lactis, Lactococcus lactis MG1363
J. Biotechnol.
134
1-8
2008
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706647
Herrero-Galan
The insecticidal protein hirsu ...
Aspergillus giganteus
Proteins
72
217 - 228
2008
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666432
Korennykh
The electrostatic character of ...
Aspergillus restrictus
Nat. Struct. Mol. Biol.
13
436-443
2006
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702502
Alvarez-Garcia
Tyr-48, a conserved residue in ...
Aspergillus giganteus
Biol. Chem.
387
535-541
2006
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664805
Garcia-Mayoral
Refined NMR structure of alpha ...
Aspergillus giganteus
Eur. Biophys. J.
34
1057-1065
2005
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666509
Petyuk
Hybridization of antisense oli ...
Escherichia coli
Nucleosides Nucleotides Nucleic Acids
23
895-906
2004
1
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646320
Masip
Leucine 145 of the ribotoxin a ...
Aspergillus giganteus
Protein Sci.
12
161-169
2003
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646321
Perez-Canadilllas
Tautomeric state of alpha-sarc ...
Aspergillus giganteus
FEBS Lett.
534
197-201
2003
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646317
Garcia-Ortega
Deletion of the NH2-terminal b ...
Aspergillus giganteus
J. Biol. Chem.
277
18632-18639
2002
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646318
Larsson
Conformational changes in the ...
Aspergillus giganteus
Biochim. Biophys. Acta
1577
53-62
2002
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646319
Liu
Purification of alpha-sarcin a ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
Protein Expr. Purif.
25
50-58
2002
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646315
Masip
Arginine 121 is a crucial resi ...
Aspergillus giganteus
Eur. J. Biochem.
268
6190-6196
2001
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646316
Olmo
Cytotoxic mechanism of the rib ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
Eur. J. Biochem.
268
2113-2123
2001
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646312
Hwu
The action mode of the ribosom ...
Aspergillus giganteus
J. Biomed. Sci.
7
420-428
2000
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646313
Perez-Canadillas
The highly refined solution st ...
Aspergillus giganteus
J. Mol. Biol.
299
1061-1073
2000
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646314
Xu
Effects of the active aldehyde ...
Aspergillus giganteus
Biol. Chem.
381
113-119
2000
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646311
Lacadena
Role of histidine-50, glutamic ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
Proteins
37
474-484
1999
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646308
Hao
Purification of alpha-sarcin a ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
Protein Expr. Purif.
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295-301
1998
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646309
Lacadena
The cytotoxin alpha-sarcin beh ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
FEBS Lett.
424
46-48
1998
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646310
Perez-Canadillas
Characterization of pKa values ...
Aspergillus giganteus
Biochemistry
37
15865-15876
1998
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1
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646307
Sylvester
Characterization of prokaryoti ...
Aspergillus giganteus
Biochim. Biophys. Acta
1358
53-60
1997
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646305
Campos-Olivas
Structural basis for the catal ...
Aspergillus giganteus
FEBS Lett.
399
163-165
1996
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646306
Cheung
Substrate specificity of monom ...
Aspergillus giganteus
FEBS Lett.
386
60-64
1996
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2
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646303
Gasset
Thermal unfolding of the cytot ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
Biochim. Biophys. Acta
1252
126-134
1995
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646304
Lacadena
Substitution of histidine-137 ...
Aspergillus giganteus, Aspergillus giganteus MDH 18894
Biochem. J.
309
581-586
1995
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646302
Ling
Cleavage of supercoiled double ...
Aspergillus giganteus
FEBS Lett.
345
143-146
1994
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646301
Stirpe
Modification of ribosomal RNA ...
Aspergillus giganteus
Nucleic Acids Res.
16
1349-1357
1988
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646300
Wool
-
The mechanism of the action of ...
Aspergillus giganteus
Trends Biochem. Sci.
9
14-17
1984
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646299
Endo
The ribonuclease activity of t ...
Aspergillus giganteus
J. Biol. Chem.
258
2662-2667
1983
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646298
Endo
The site of action of alpha-sa ...
Aspergillus giganteus
J. Biol. Chem.
257
9054-9060
1982
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