BRENDA - Reference to 3.1.27.10; Id = 646312

cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template

Aspergillus giganteus

C148S

point mutation constructed from cDNA clone

Aspergillus giganteus

C6S

point mutation constructed from cDNA clone

Aspergillus giganteus

rRNA + H2O

Aspergillus giganteus

-

?

-

?

Aspergillus giganteus

-

filamentous fungus, mold

-

recombinant enzyme

Aspergillus giganteus

RNA + H2O

hydrolyzes naked RNA at purines in both single-and double-stranded regions

646312

Aspergillus giganteus

?

-

?

rRNA + H2O

-

646312

Aspergillus giganteus

?

-

?

cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template

Aspergillus giganteus

C148S

point mutation constructed from cDNA clone

Aspergillus giganteus

C6S

point mutation constructed from cDNA clone

Aspergillus giganteus

rRNA + H2O

Aspergillus giganteus

-

?

-

?

recombinant enzyme

Aspergillus giganteus

RNA + H2O

hydrolyzes naked RNA at purines in both single-and double-stranded regions

646312

Aspergillus giganteus

?

-

?

rRNA + H2O

-

646312

Aspergillus giganteus

?

-

?

730369

Alford

Conditional protein splicing o ...

Aspergillus giganteus

Mol. Biosyst.

10

831-837

2014

-

1

-

3

-

1

-

1

-

3

-

1

-

730862

Carreras-Sangra

Production and characterizatio ...

Aspergillus giganteus, Aspergillus giganteus MDH18894

Protein Eng. Des. Sel.

25

425-435

2012

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

730992

He

Mechanisms for ribotoxin-induc ...

Aspergillus giganteus

Toxicol. Appl. Pharmacol.

265

10-18

2012

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

2

-

710149

Garcia-Ortega

Cleavage of the sarcin-ricin l ...

Aspergillus giganteus

Nucleic Acids Res.

38

4108-4119

2010

-

1

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

690631

Alvarez-Garcia

Role of the basic character of ...

Fungi

Arch. Biochem. Biophys.

481

37-44

2009

-

1

-

5

-

1

-

1

-

5

-

707861

Alford

alpha-Sarcin catalytic activit ...

Aspergillus giganteus

BMC Biochem.

10

9-9

2009

-

1

-

2

-

2

-

2

-

1

-

2

-

2

-

2

-

683339

Jandhyala

ZAK: a MAP3Kinase that transdu ...

Escherichia coli

Cell. Microbiol.

10

1468-1477

2008

-

2

-

1

-

1

-

704754

Alvarez-Garcia

Lactococcus lactis as a vehicl ...

Lactococcus lactis, Lactococcus lactis MG1363

J. Biotechnol.

134

1-8

2008

-

1

-

10

-

1

-

1

-

1

-

706647

Herrero-Galan

The insecticidal protein hirsu ...

Aspergillus giganteus

Proteins

72

217 - 228

2008

-

2

-

1

-

4

-

2

-

2

-

2

-

1

-

2

-

2

-

666432

Korennykh

The electrostatic character of ...

Aspergillus restrictus

Nat. Struct. Mol. Biol.

13

436-443

2006

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

702502

Alvarez-Garcia

Tyr-48, a conserved residue in ...

Aspergillus giganteus

Biol. Chem.

387

535-541

2006

-

1

-

2

-

1

-

1

-

2

-

1

-

2

-

1

-

2

-

664805

Garcia-Mayoral

Refined NMR structure of alpha ...

Aspergillus giganteus

Eur. Biophys. J.

34

1057-1065

2005

-

1

-

1

-

1

-

1

-

1

-

666509

Petyuk

Hybridization of antisense oli ...

Escherichia coli

Nucleosides Nucleotides Nucleic Acids

23

895-906

2004

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

646320

Masip

Leucine 145 of the ribotoxin a ...

Aspergillus giganteus

Protein Sci.

12

161-169

2003

-

1

-

1

-

2

-

1

-

1

-

1

-

3

-

1

2

1

-

1

-

1

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2

-

1

-

1

-

3

-

1

2

1

-

646321

Perez-Canadilllas

Tautomeric state of alpha-sarc ...

Aspergillus giganteus

FEBS Lett.

534

197-201

2003

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

646317

Garcia-Ortega

Deletion of the NH2-terminal b ...

Aspergillus giganteus

J. Biol. Chem.

277

18632-18639

2002

-

1

-

1

-

1

-

3

-

1

-

4

-

1

-

1

-

1

-

1

-

4

-

646318

Larsson

Conformational changes in the ...

Aspergillus giganteus

Biochim. Biophys. Acta

1577

53-62

2002

-

1

-

2

-

2

-

1

-

2

-

646319

Liu

Purification of alpha-sarcin a ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

Protein Expr. Purif.

25

50-58

2002

-

2

-

8

-

1

-

1

-

2

-

2

-

1

-

1

-

2

-

646315

Masip

Arginine 121 is a crucial resi ...

Aspergillus giganteus

Eur. J. Biochem.

268

6190-6196

2001

-

1

-

2

-

3

1

-

1

-

2

-

1

-

5

-

1

-

3

-

1

-

2

-

3

1

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1

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1

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5

-

1

-

3

-

646316

Olmo

Cytotoxic mechanism of the rib ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

Eur. J. Biochem.

268

2113-2123

2001

-

1

-

2

-

9

-

1

-

6

-

1

-

2

-

1

-

6

-

646312

Hwu

The action mode of the ribosom ...

Aspergillus giganteus

J. Biomed. Sci.

7

420-428

2000

-

1

-

2

-

1

-

1

-

1

-

2

-

1

-

2

-

1

-

1

-

2

-

646313

Perez-Canadillas

The highly refined solution st ...

Aspergillus giganteus

J. Mol. Biol.

299

1061-1073

2000

-

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

1

-

2

-

646314

Xu

Effects of the active aldehyde ...

Aspergillus giganteus

Biol. Chem.

381

113-119

2000

-

1

-

1

-

2

-

1

-

2

-

646311

Lacadena

Role of histidine-50, glutamic ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

Proteins

37

474-484

1999

-

1

-

5

-

3

1

-

2

-

9

-

1

-

6

-

4

1

-

1

-

5

-

3

1

-

2

-

1

-

6

-

4

1

-

646308

Hao

Purification of alpha-sarcin a ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

Protein Expr. Purif.

14

295-301

1998

-

1

-

2

-

10

-

1

-

6

-

1

-

2

-

1

-

6

-

646309

Lacadena

The cytotoxin alpha-sarcin beh ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

FEBS Lett.

424

46-48

1998

-

2

-

2

-

8

-

8

-

2

-

2

-

2

-

8

-

2

-

646310

Perez-Canadillas

Characterization of pKa values ...

Aspergillus giganteus

Biochemistry

37

15865-15876

1998

-

1

-

1

-

2

-

1

2

1

-

1

-

2

-

1

2

1

-

646307

Sylvester

Characterization of prokaryoti ...

Aspergillus giganteus

Biochim. Biophys. Acta

1358

53-60

1997

-

1

-

1

-

1

-

3

-

1

-

3

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1

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1

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1

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1

-

3

-

646305

Campos-Olivas

Structural basis for the catal ...

Aspergillus giganteus

FEBS Lett.

399

163-165

1996

-

1

-

1

-

2

-

1

-

2

-

646306

Cheung

Substrate specificity of monom ...

Aspergillus giganteus

FEBS Lett.

386

60-64

1996

-

1

-

1

-

1

-

5

2

-

1

-

1

-

5

2

-

646303

Gasset

Thermal unfolding of the cytot ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

Biochim. Biophys. Acta

1252

126-134

1995

-

1

-

1

2

-

8

-

1

-

4

-

1

-

1

-

1

2

-

1

-

4

-

1

-

646304

Lacadena

Substitution of histidine-137 ...

Aspergillus giganteus, Aspergillus giganteus MDH 18894

Biochem. J.

309

581-586

1995

-

1

-

2

-

1

-

2

-

9

-

1

-

6

-

1

-

2

-

1

-

2

-

1

-

6

-

646302

Ling

Cleavage of supercoiled double ...

Aspergillus giganteus

FEBS Lett.

345

143-146

1994

-

1

-

1

-

2

-

1

-

2

-

646301

Stirpe

Modification of ribosomal RNA ...

Aspergillus giganteus

Nucleic Acids Res.

16

1349-1357

1988

-

1

-

1

-

2

-

1

-

2

-

646300

Wool

-

The mechanism of the action of ...

Aspergillus giganteus

Trends Biochem. Sci.

9

14-17

1984

-

1

-

1

-

1

-

5

-

1

-

1

-

1

-

5

-

1

-

646299

Endo

The ribonuclease activity of t ...

Aspergillus giganteus

J. Biol. Chem.

258

2662-2667

1983

-

1

-

1

-

1

-

2

-

3

-

1

-

1

-

1

-

1

-

3

-

1

-

646298

Endo

The site of action of alpha-sa ...

Aspergillus giganteus

J. Biol. Chem.

257

9054-9060

1982

-

1

-

1

-

4

-

4

-

1

-

1

-

4

-

The action mode of the ribosome-inactivating protein alpha-sarcin

Data extracted from this reference: