Application | Comment | Organism |
---|---|---|
medicine | Acinetobacter baumannii is a problematic pathogen that is commonly resistant to multiple antibiotics. The RNase P endonucleolytic activity is characterized by having structural but not sequence substrate requirements. This property leads to development of EGS technology, which consists of utilizing a short antisense oligonucleotide that when forming a duplex with a target RNA induces its cleavage by RNase P. External guide sequence (EGS) technology can be a viable option for designing therapeutic alternatives to treat multiresistant Acinetobacter baumannii infections | Acinetobacter baumannii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Acinetobacter baumannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | B0V5R2 | consists of a catalytic RNA component (M1 or rnpB) and B0V5R2: Ribonuclease P protein component | - |
Acinetobacter baumannii ATCC 17978 | B0V5R2 | consists of a catalytic RNA component (M1 or rnpB) and B0V5R2: Ribonuclease P protein component | - |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | the RNase P endonucleolytic activity is characterized by having structural but not sequence substrate requirements. This property leads to development of EGS technology, which utilizes a short antisense oligonucleotide that when forming a duplex with a target RNA induces its cleavage by RNase P | Acinetobacter baumannii | mature tRNA + 5'-terminal oligonucleotide | - |
? | |
pre-tRNA + H2O | the RNase P endonucleolytic activity is characterized by having structural but not sequence substrate requirements. This property leads to development of EGS technology, which utilizes a short antisense oligonucleotide that when forming a duplex with a target RNA induces its cleavage by RNase P | Acinetobacter baumannii ATCC 17978 | mature tRNA + 5'-terminal oligonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Acinetobacter baumannii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved maturation of tRNAs by endonucleolytic cleavage of the pre-tRNA | Acinetobacter baumannii |