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Literature summary for 3.1.26.5 extracted from

  • Davies-Sala, C.; Jani, S.; Zorreguieta, A.; Tolmasky, M.E.
    Identification of the Acinetobacter baumannii ribonuclease P catalytic subunit cleavage of a target mRNA in the presence of an external guide sequence (2018), Front. Microbiol., 9, 2408 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine Acinetobacter baumannii is a problematic pathogen that is commonly resistant to multiple antibiotics. The RNase P endonucleolytic activity is characterized by having structural but not sequence substrate requirements. This property leads to development of EGS technology, which consists of utilizing a short antisense oligonucleotide that when forming a duplex with a target RNA induces its cleavage by RNase P. External guide sequence (EGS) technology can be a viable option for designing therapeutic alternatives to treat multiresistant Acinetobacter baumannii infections Acinetobacter baumannii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Acinetobacter baumannii

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii B0V5R2 consists of a catalytic RNA component (M1 or rnpB) and B0V5R2: Ribonuclease P protein component
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Acinetobacter baumannii ATCC 17978 B0V5R2 consists of a catalytic RNA component (M1 or rnpB) and B0V5R2: Ribonuclease P protein component
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Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA + H2O the RNase P endonucleolytic activity is characterized by having structural but not sequence substrate requirements. This property leads to development of EGS technology, which utilizes a short antisense oligonucleotide that when forming a duplex with a target RNA induces its cleavage by RNase P Acinetobacter baumannii mature tRNA + 5'-terminal oligonucleotide
-
?
pre-tRNA + H2O the RNase P endonucleolytic activity is characterized by having structural but not sequence substrate requirements. This property leads to development of EGS technology, which utilizes a short antisense oligonucleotide that when forming a duplex with a target RNA induces its cleavage by RNase P Acinetobacter baumannii ATCC 17978 mature tRNA + 5'-terminal oligonucleotide
-
?

Synonyms

Synonyms Comment Organism
RNase P
-
Acinetobacter baumannii

General Information

General Information Comment Organism
physiological function the enzyme is involved maturation of tRNAs by endonucleolytic cleavage of the pre-tRNA Acinetobacter baumannii