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Literature summary for 3.1.26.5 extracted from

  • Derksen, M.; Mertens, V.; Pruijn, G.J.
    RNase P-mediated sequence-specific cleavage of RNA by engineered external guide sequences (2015), Biomolecules, 5, 3029-3050 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Application

Application Comment Organism
medicine two different technologies are developed to use RNase P as a tool for RNA knockdown. In one of these, an external guide sequence (EGS), which mimics a tRNA precursor, a well-known natural RNase P substrate, is used to target an RNA molecule for cleavage by endogenous RNase P. Alternatively, a guide sequence can be attached to M1 RNA, the (catalytic) RNase P RNA subunit of Escherichia coli. The guide sequence is specific for an RNA target, which is subsequently cleaved by the bacterial M1 RNA moiety. These approaches are applicable in both bacteria and eukaryotes. The RNase P EGS and M1GS technologies to knockdown specific RNAs are useful strategy in combating infectious diseases. External guide sequence (EGS) and M1 guide sequence (M1GS) successfully reduced bacterial viability, malaria replication and viral infections in cell cultures, and, to a lesser extent, in multicellular organisms. Salmonella-based oral delivery of EGS and M1GS appears to be an attractive way to combat some viral infections in mice and possibly other organisms Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Synonyms

Synonyms Comment Organism
RNase P
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 Escherichia coli

General Information

General Information Comment Organism
physiological function the enzyme removes the 5'-leader sequence from tRNA precursors Escherichia coli