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Literature summary for 3.1.26.5 extracted from

  • Zeng, W.; Chen, Y.C.; Bai, Y.; Trang, P.; Vu, G.P.; Lu, S.; Wu, J.; Liu, F.
    Effective inhibition of human immunodeficiency virus 1 replication by engineered RNase P ribozyme (2012), PLoS ONE, 7, e51855.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Application

Application Comment Organism
pharmacology engineered Escherichia coli ribozyme variants are effective in inhibiting HIV infection, the potential of engineering RNase P ribozymes for anti-HIV application Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
cloning of mutant enzyme in amphotropic packaging PA317 cells, heterologous expression of wild-type and mutant enzymes in human H9 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a mutant variant M1GS ribozyme via combined mutations at nucleotide 83 and 340 of RNase P catalytic RNA (G83 -. U83 and G340 -. A340). The mutant ribozyme shows an increase in overall efficiency in cleaving an HIV RNA sequence compared to the wild-type enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the natural substrate is precursor tRNA ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the natural substrate is precursor tRNA Escherichia coli ?
-
 ?
additional information construction of a modell substrate for M1 RNA from Escherichia coli. wild-type and mutant enzymes cleave the HIV RNA sequence in the tat region. The variant, containing combined mutations at nucleotide 83 and 340 of RNase P catalytic RNA, cleaves the tat RNA sequence in vitro about 20 times more efficiently than the wild-type ribozyme Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
RNase P
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

General Information

General Information Comment Organism
physiological function the mutant enzyme variant is more effective in HIV RNA sequence cleavage and reducing HIV-1 p24 expression and intracellular viral RNA level in cells than the wild-type ribozyme. A reduction of about 90% in viral RNA level and a reduction of 150fold in viral growth are observed in human H9 cells that express the mutant, while a reduction of less than 10% is observed in H9 cells that either do not express the ribozyme or produce a catalytically inactive ribozyme mutant Escherichia coli