Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.5 extracted from

  • Bai, Y.; Gong, H.; Li, H.; Vu, G.P.; Lu, S.; Liu, F.
    Oral delivery of RNase P ribozymes by Salmonella inhibits viral infection in mice (2011), Proc. Natl. Acad. Sci. USA, 108, 3222-3227.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Protein Variants

Protein Variants Comment Organism
additional information construction of a functional RNase P-based ribozyme, M1GS RNA, that targets the overlapping mRNA region of M80.5 and protease, two murine cytomegalovirus proteins essential for viral replication. Construction of an attenuated strain of Salmonella, which exhibits efficient gene transfer activity and little cytotoxicity and pathogenicity in mice, for delivery of anti-MCMV ribozyme. In MCMV-infected macrophages treated with the constructed attenuated Salmonella strain carrying the functional M1GS RNA construct, a 80-85% reduction in the expression of M80.5/protease and a 2500fold reduction in viral growth is observed. The strain delivers efficiently delivered antiviral M1GS RNA into spleens and livers, leading to substantial expression of the ribozyme without causing significant adverse effects in the animals. MCMV-infected mice treated orally with Salmonella carrying the functional M1GS sequence display reduced viral gene expression, decreased viral titers, and improved survival compared to the untreated mice or mice treated with Salmonella containing control ribozyme sequences Salmonella sp.

Organism

Organism UniProt Comment Textmining
Salmonella sp.
-
-
-

Synonyms

Synonyms Comment Organism
RNase P
-
 Salmonella sp.