Cloned (Comment) | Organism |
---|---|
pET-15b-derived plasmid endcoding the C5 protein expressed in Escherichia coli BL21(DE3). Into pUC19 vector and transformed into Escherichia coli strain JM109 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activation | Escherichia coli | |
Mg2+ | required for activation | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | is unable to activate non-cognate RNase P RNAs, Pyrococcus horikoshii RNase P RNA and Escherichia coli RNase P RNA. Chimeric RNase P RNAs composed of the Escherichia coli RNA C-domain and Pyrococcus horikoshii S-domain or composed of the Pyrococcus horikoshii C-domain and Escherichia coli RNA S-domain, respectively, exhibit activity. C5 protein is involved in activation of the Escherichia coli pRNA C-domain | Escherichia coli | ? | - |
? | |
additional information | RNase P proteins Pop5, Rpp21, Rpp29, Rpp30 and Rpp38 are unable to activate non-cognate RNase P RNAs, Pyrococcus horikoshii RNase P RNA and Escherichia coli RNase P RNA. Chimeric RNase P RNAs composed of the Escherichia coli RNA C-domain and Pyrococcus horikoshii S-domain or composed of the Pyrococcus horikoshii C-domain and Escherichia coli RNA S-domain, respectively, exhibit activity. Pop5 and Rpp30 are involved in activation of the Pyrococcus horikoshii pRNA C-domain, whereas Rpp21 and Rpp29 are implicated in stabilization of the Pyrococcus horikoshii pRNA S-domain | Pyrococcus horikoshii | ? | - |
? | |
pre-tRNATyr + H2O | cleaves efficiently at a single phosphodieste bond between positions U59 and C60 | Escherichia coli | mature tRNATyr + 5'-oligoribonucleotide | - |
? | |
pre-tRNATyr + H2O | is completely processed. Cleaves efficiently at a single phosphodieste bond between positions U59 and C60 | Pyrococcus horikoshii | mature tRNATyr + 5'-oligoribonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pop5 | - |
Pyrococcus horikoshii |
Protein C5 | - |
Escherichia coli |
ribonuclease P | - |
Escherichia coli |
ribonuclease P | - |
Pyrococcus horikoshii |
RNase P | - |
Escherichia coli |
RNase P | - |
Pyrococcus horikoshii |
Rpp21 | - |
Pyrococcus horikoshii |
Rpp29 | - |
Pyrococcus horikoshii |
Rpp30 | - |
Pyrococcus horikoshii |
Rpp38 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
50 | - |
- |
Pyrococcus horikoshii |