Cloned (Comment) | Organism |
---|---|
full-length Pop6 and Pop7 coexpressed in Escherichia coli. Selenomethionine derivative of the Pop6-Pop7 heterodimer obtained using Escherichia coli strain BL21 | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
complex including a circularly permuted 46-nucleotide-long P3 domain of the RNA component of ribonuclease MRP and selenomethionine derivatives of the shared ribonuclease P/MRP protein components Pop6 and Pop7, using the sitting-drop vapour-diffusion method. The crystals belong to space group P4222 with unit-cell parameters a = b = 127.2, c = 76.8 A , alpha = beta = gamma = 90° and diffract to 3.25 A resolution. The terminal part of the proximal helical stem of the P3 domain is not involved in interactions with Pop6-Pop7 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for formation of the Pop6-Pop7-RNA complex | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Pop6 and Pop7 | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, 10 mM Tris-HCl buffer, pH 7.4, 100 mM NaCl, 50 mM KCl, 2 mM DTT, 0.1 mM Na EDTA and 0.1 mM PMSF, several weeks | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Pop6 | - |
Saccharomyces cerevisiae |
Pop7 | - |
Saccharomyces cerevisiae |
ribonuclease MRP | - |
Saccharomyces cerevisiae |
ribonuclease P | - |
Saccharomyces cerevisiae |
RNase P | - |
Saccharomyces cerevisiae |
RNase P/MRP protein | - |
Saccharomyces cerevisiae |