Crystallization (Comment) | Organism |
---|---|
the P7DELTA RNA which is comprised of the independently folding catalytic domain of the RNase P RNA is crystallized by vapour diffusion method with 50 mM Na-cacodylate at pH 6.5, 100 mM KCL, 0.7-1.0 mM spermidine tetrachloride, and 21-23% 1,6-hexanediol | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for folding, substrate binding, and catalysis | Geobacillus stearothermophilus | |
additional information | Mn2+, Co2+, Ni2+, Cu2+, Au3+, Pb2+, La3+, Pr3+, Sm3+, Gd3+, Dy3+, Yb3+, and Lu3+ are able to bind to RNase P but are not specific proxies for Mg2+ | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA + H2O | - |
Geobacillus stearothermophilus | tRNA + 5'-oligoribonucleotide | - |
? | |
pre-tRNA precursor + H2O | - |
Geobacillus stearothermophilus | tRNA + 5'-oligoribonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | - |
Geobacillus stearothermophilus |