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Literature summary for 3.1.26.5 extracted from

  • Hsieh, J.; Walker, S.C.; Fierke, C.A.; Engelke, D.R.
    Pre-tRNA turnover catalyzed by the yeast nuclear RNase P holoenzyme is limited by product release (2009), RNA, 15, 224-234.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00006
-
pre-tRNA-Tyr pre-tRNA-Tyr without the 3'-UUUUU trailer, at 25°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl Saccharomyces cerevisiae
0.0001
-
pre-tRNA-Tyr pre-tRNA-Tyr with the 3'-UUUUU trailer, at 25°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae SCWY10
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein
-
Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
tandem affinity purification tag chromatography, calmodulin affinity resin chromatography, and DEAE Sepharose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA-Tyr + H2O
-
Saccharomyces cerevisiae tRNA-Tyr + 5'-oligoribonucleotide
-
?
pre-tRNA-Tyr + H2O
-
Saccharomyces cerevisiae SCWY10 tRNA-Tyr + 5'-oligoribonucleotide
-
?

Synonyms

Synonyms Comment Organism
RNase P yeast RNase P holoenzyme comprises one RNA subunit and nine protein subunits Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.077
-
pre-tRNA-Tyr pre-tRNA-Tyr without the 3'-UUUUU trailer, at 20°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl Saccharomyces cerevisiae
0.08
-
pre-tRNA-Tyr pre-tRNA-Tyr with the 3'-UUUUU trailer, at 20°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl Saccharomyces cerevisiae