KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
pre-tRNA-Tyr | pre-tRNA-Tyr without the 3'-UUUUU trailer, at 25°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl | Saccharomyces cerevisiae | |
0.0001 | - |
pre-tRNA-Tyr | pre-tRNA-Tyr with the 3'-UUUUU trailer, at 25°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae SCWY10 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
tandem affinity purification tag chromatography, calmodulin affinity resin chromatography, and DEAE Sepharose column chromatography | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA-Tyr + H2O | - |
Saccharomyces cerevisiae | tRNA-Tyr + 5'-oligoribonucleotide | - |
? | |
pre-tRNA-Tyr + H2O | - |
Saccharomyces cerevisiae SCWY10 | tRNA-Tyr + 5'-oligoribonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | yeast RNase P holoenzyme comprises one RNA subunit and nine protein subunits | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
pre-tRNA-Tyr | pre-tRNA-Tyr without the 3'-UUUUU trailer, at 20°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl | Saccharomyces cerevisiae | |
0.08 | - |
pre-tRNA-Tyr | pre-tRNA-Tyr with the 3'-UUUUU trailer, at 20°C, pH 8.0, in 10 mM HEPES buffer with 10 mM MgCl2 and 100 mM KCl | Saccharomyces cerevisiae |