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Literature summary for 3.1.26.5 extracted from

  • Honda, T.; Kakuta, Y.; Kimura, K.; Saho, J.; Kimura, M.
    Structure of an archaeal homolog of the human protein complex Rpp21-Rpp29 that is a key core component for the assembly of active ribonuclease P (2008), J. Mol. Biol., 384, 652-662.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
PhoRpp21 and PhoRpp29, hanging drop vapour diffusion method. using 50 mM Tris-HCl (pH 7.5) containing 50 mM sodium chloride, 200 mM potassium nitrate, and 20% w/v PEG 3350 Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
E47A mutant shows activity similar to the wild type enzyme Pyrococcus horikoshii
E73A mutant shows reduced activity compared to the wild type enzyme Pyrococcus horikoshii
F95A mutant shows reduced activity compared to the wild type enzyme Pyrococcus horikoshii
K121A mutant shows activity similar to the wild type enzyme Pyrococcus horikoshii
K122A mutant shows activity similar to the wild type enzyme Pyrococcus horikoshii
K90A mutant shows strongly reduced activity compared to the wild type enzyme Pyrococcus horikoshii
R115A mutant shows reduced activity compared to the wild type enzyme Pyrococcus horikoshii
R75A mutant shows reduced activity compared to the wild type enzyme Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59425
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein
-
Pyrococcus horikoshii

Purification (Commentary)

Purification (Comment) Organism
SP-Sepharose column chromatography Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA precursor + H2O
-
Pyrococcus horikoshii tRNA + 5'-oligoribonucleotide
-
?

Synonyms

Synonyms Comment Organism
RNase P contains catalytic RNA and the RNase P proteins PhoRpp21 and PhoRpp29 Pyrococcus horikoshii