Crystallization (Comment) | Organism |
---|---|
PhoRpp21 and PhoRpp29, hanging drop vapour diffusion method. using 50 mM Tris-HCl (pH 7.5) containing 50 mM sodium chloride, 200 mM potassium nitrate, and 20% w/v PEG 3350 | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
E47A | mutant shows activity similar to the wild type enzyme | Pyrococcus horikoshii |
E73A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
F95A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
K121A | mutant shows activity similar to the wild type enzyme | Pyrococcus horikoshii |
K122A | mutant shows activity similar to the wild type enzyme | Pyrococcus horikoshii |
K90A | mutant shows strongly reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
R115A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
R75A | mutant shows reduced activity compared to the wild type enzyme | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O59425 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
ribonucleoprotein | - |
Pyrococcus horikoshii |
Purification (Comment) | Organism |
---|---|
SP-Sepharose column chromatography | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pre-tRNA precursor + H2O | - |
Pyrococcus horikoshii | tRNA + 5'-oligoribonucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase P | contains catalytic RNA and the RNase P proteins PhoRpp21 and PhoRpp29 | Pyrococcus horikoshii |