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Literature summary for 3.1.26.5 extracted from
- Functional reconstitution and characterization of Pyrococcus furiosus RNase P (2006), Proc. Natl. Acad. Sci. USA, 103, 16147-16152.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli BL21(DE3) Rosetta cells | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00018 | - |
ptRNATyr | from Escherichia coli, enzyme subunit RNase P RNA + Pop5 + RNase P protein 30 + RNase P protein 21 + RNase P protein 29 | Pyrococcus furiosus | |
| 0.00653 | - |
ptRNATyr | enzyme subunit RNase P RNA + RNase P protein 21 + RNase P protein 29 | Pyrococcus furiosus | |
| 0.0116 | - |
ptRNATyr | from Escherichia coli, emzyme subunit RNase P RNA + Pop5 + RNase P protein 30 | Pyrococcus furiosus | |
| 0.0305 | - |
ptRNATyr | from Escherichia coli, enzyme substrate Rnase P RNA | Pyrococcus furiosus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | optimal 120 mM | Pyrococcus furiosus |  |
| Mg2+ | optimal 120 mM RNase P RNA + RNase P protein 21 + RNase P protein 29 | Pyrococcus furiosus | |
| Mg2+ | optimal 30 mM | Pyrococcus furiosus | |
| Mg2+ | optimal 500 mM | Pyrococcus furiosus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| precursor tRNA + H2O | Pyrococcus furiosus | catalyzes the magnesium-dependent 5'-end maturation of tRNAs | ? | - |
? | |
| ptRNATyr + H2O | Pyrococcus furiosus | from Escherichia coli | ? | - |
? | |
| ptRNATyr + H2O | Pyrococcus furiosus | from Eschericha coli | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to homogeneity by using cation-exchange and reversed-phase chromatography | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| precursor tRNA + H2O | catalyzes the magnesium-dependent 5'-end maturation of tRNAs | Pyrococcus furiosus | ? | - |
? | |
| ptRNATyr + H2O | from Escherichia coli | Pyrococcus furiosus | ? | - |
? | |
| ptRNATyr + H2O | from Eschericha coli | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | RNase P contains an essential RNase P RNA and RNase P protein | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ribunuclease P | RNase P | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0063 | - |
ptRNATyr | from Escherichia coli, enzyme substrate Rnase P RNA | Pyrococcus furiosus | |
| 0.01 | - |
ptRNATyr | enzyme subunit RNase P RNA + RNase P protein 21 + RNase P protein 29 | Pyrococcus furiosus | |
| 0.1583 | - |
ptRNATyr | from Escherichia coli, enzyme subunit RNase P RNA + Pop5 + RNase P protein 30 + RNase P protein 21 + RNase P protein 29 | Pyrococcus furiosus | |
| 0.1917 | - |
ptRNATyr | from Escherichia coli, emzyme subunit RNase P RNA + Pop5 + RNase P protein 30 | Pyrococcus furiosus |
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