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Literature summary for 3.1.26.5 extracted from

  • Zahler, N.H.; Christian, E.L.; Harris, M.E.
    Recognition of the 5' leader of pre-tRNA substrates by the active site of ribonuclease P (2003), RNA, 9, 734-745.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information binding and catalysis of J5/15 and N(-1) mutants, overview, mutation of the A248 nucleotide results in defects in thermodynamics and in miscleavage of substrates containing 2'deoxy modification at N(-1), compensatory mutation of N(-1) restore correct cleavage activity in reaction with both, the RNA subunit alone and the holoenzyme, influence of nucleotide at position N(-2) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, conserved residues in J5/15 are responsible for substrate affinity and specificity of the ribozyme, nucleotide base N(-1) is involved in substrate recognition in bacteria and archea Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, the 5'leader of the pre-tRNA substrate is recognized by the active site of the enzyme via interaction of N(-1) substrate nucleotide with A248 of the ribozyme, preference for U at position N(-1) Escherichia coli mature tRNA + 5'-oligonucleotide generates 5'-phosphate,3'-hydroxyl-product ?

Subunits

Subunits Comment Organism
More enzyme is composed of a large RNA subunit of about 400 nucleotides and a smaller protein subunit Escherichia coli

Synonyms

Synonyms Comment Organism
RNase P
-
 Escherichia coli
tRNA processing enzyme
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli