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Literature summary for 3.1.26.5 extracted from

  • Xiao, S.; Houser-Scott, F.; Engelke, D.R.
    Eukaryotic ribonuclease P: increased complexity to cope with the nuclear pre-tRNA pathway (2001), J. Cell. Physiol., 187, 11-20.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.5

Protein Variants

Protein Variants Comment Organism
additional information construction of mutants inactive with pre-4.5S RNA but not with pre-tRNA Bacteria

Inhibitors

Inhibitors Comment Organism Structure
EDTA inactivation Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information both pre-steady state and steady state kinetics Bacteria
additional information
-
 additional information pre-steady state kinetics, nuclear enzyme form Saccharomyces cerevisiae
0.000055
-
 tRNA precursor nuclear enzyme form Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast enzyme form might be RNA-independent Spinacia oleracea 9507
-
mitochondrion mitochondrial enzyme form of RNase MRP Homo sapiens 5739
-
mitochondrion mitochondrial ribozyme form Saccharomyces cerevisiae 5739
-
additional information subcellular localization of different enzyme forms Homo sapiens
-
-
additional information subcellular localization of different enzyme forms Saccharomyces cerevisiae
-
-
nucleolus nucleolar enzyme form of RNase MRP Homo sapiens 5730
-
nucleus nuclear ribozyme form RNase P in nucleoplasm Homo sapiens 5634
-
nucleus nuclear ribozyme forms MPR and P Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ high activation Bacteria
Mg2+ high activation Bacillus subtilis
Mg2+ high activation Homo sapiens
Mg2+ high activation, specific for Saccharomyces cerevisiae
Mn2+ can substitute for Mg2+, slightly lower activity Bacteria
Mn2+ can substitute for Mg2+, slightly lower activity Bacillus subtilis
Mn2+ can substitute for Mg2+, slightly lower activity Homo sapiens
Mn2+ can substitute for Mg2+, slightly lower activity Saccharomyces cerevisiae
additional information divalent metal ions are absolutely required, reduced activity with Ca2+ Bacteria
additional information divalent metal ions are absolutely required, reduced activity with Ca2+ Bacillus subtilis
additional information divalent metal ions are absolutely required, reduced activity with Ca2+ Homo sapiens
additional information divalent metal ions are absolutely required, reduced activity with Ca2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15500
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
15800
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
16300
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
18200
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
19600
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
22500
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
22600
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
32200
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
32900
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
100500
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
112000
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
120000
-
 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10Sa RNA + H2O Bacteria
-
 ?
-
 ?
4.55 rRNA precursor + H2O Bacteria
-
 mature 4.55 rRNA + 5'-oligonucleotide
-
 ?
additional information Saccharomyces cerevisiae the mitochondrial ribozyme RNA subunit might have a cellular function outside the mitochondria ?
-
 ?
polycistronic his operon mRNA precursor + H2O Bacteria
-
 ?
-
 ?
tRNA precursor + H2O Cyanophora paradoxa cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Bacteria cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Bacillus subtilis cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Homo sapiens cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Saccharomyces cerevisiae cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O Spinacia oleracea cleavage of 5'-terminal oligonucleotide, maturation mature tRNA + 5'-oligonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 enzyme is a catalytically active RNA with only a small additional protein subunit
-
Bacteria
-
 enzyme is a catalytically active RNA, at least 2 isoforms with different substrate specificities
-
Cyanophora paradoxa
-
-
-
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
-
-
Spinacia oleracea
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein enzyme is a ribonucleoprotein with a large protein part and 1 RNA subunit Cyanophora paradoxa
ribonucleoprotein enzyme is a ribonucleoprotein with a large protein part and 1 RNA subunit Homo sapiens
ribonucleoprotein enzyme is a ribonucleoprotein with a large protein part and 1 RNA subunit Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae
partially Spinacia oleracea

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, non-RNA-based cleavage mechanism, structure-function relationship of eukaryotic enzymes Spinacia oleracea
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, reaction mechanism in 3 consecutive steps: pre-tRNA binding, hydrolysis of the scissile phosphodiester bond, generating a 3'-hydroxyl and a 5'-phosphate group, possibly via a trigonal bipyramide, intermediate, and the dissociation of the mature tRNA and the 5'-leader product, structure-function relationship of eukaryotic enzymes Homo sapiens
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, reaction mechanism in 3 consecutive steps: pre-tRNA binding, hydrolysis of the scissile phosphodiester bond, generating a 3'-hydroxyl and a 5'-phosphate group, possibly via a trigonal bipyramide, intermediate, and the dissociation of the mature tRNA and the 5'-leader product, substrate recognition mechanism, overview, structure-function realtionship of eukaryotic enzymes Saccharomyces cerevisiae
a
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor an RNA-containing enzyme, essential for tRNA processing, generates 5'-termini or mature tRNA molecules, substrate recognition, enzyme possesses a single-stranded RNA binding cleft that interacts with the unpaired 5'-leader Bacillus subtilis
a

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-
leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10Sa RNA + H2O
-
 Bacteria ?
-
 ?
4.55 rRNA precursor + H2O
-
 Bacteria mature 4.55 rRNA + 5'-oligonucleotide
-
 ?
additional information the mitochondrial ribozyme RNA subunit might have a cellular function outside the mitochondria Saccharomyces cerevisiae ?
-
 ?
additional information substrate specificities of enzyme forms RNase P and RNase MRP Homo sapiens ?
-
 ?
additional information substrate specificities of enzyme forms RNase P and RNase MRP Saccharomyces cerevisiae ?
-
 ?
polycistronic his operon mRNA precursor + H2O
-
 Bacteria ?
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Cyanophora paradoxa mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Bacteria mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Bacillus subtilis mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Homo sapiens mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Saccharomyces cerevisiae mature tRNA + 5'-oligonucleotide
-
 ?
tRNA precursor + H2O cleavage of 5'-terminal oligonucleotide, maturation Spinacia oleracea mature tRNA + 5'-oligonucleotide
-
 ?

Subunits

Subunits Comment Organism
decamer 1 * 120000, RNA subunit, + 1 * 100500, protein subunit Pop1p, + 1 * 22600, protein subunit Pop3p, 1 * 32900, protein subunit Pop4p, + 1 * 19600, protein subunit Pop5p, + 1 * 18200, protein subunit Pop6p, + 1 * 15800, protein subunit Pop7p, + 1 * 15500, protein subunit Pop8p, + 1 * 32200, protein subunit Rpp1p, + 1 * 16300, protein subunit Rpr2p, or 1 * 112000, RNA subunit NME1, + 1 * 22500, protein subunit SNM1 Saccharomyces cerevisiae
More subunit composition for RNase P: 1 RNA subunit RPM1 plus at least 1 protein subunit Rpm2p for the mitochondrial ribozyme, 9 protein components for the nuclear enzyme form, namely Pop1p, Pop3p-Pop8p, Rpp1p, and Rpr2p, secondary structure of nuclear enzyme RNA, subunit composition for RNase MRP: 1 RNA subunit NME1 plus 9 protein components for the nuclear enzyme form, namely Pop1p, Pop3p-Pop7p, Pop, Rpp1p, and SNM1, overview, Pop7p is also known as Rpp2p Saccharomyces cerevisiae
oligomer subunit composition, 1 RNA subunit, i.e. H1 for RNase P or 7-2 for RNase MRP, plus 9 protein components namely Pop1p, Rpp29p, Rpp20p, Rpp30p, Rep38p, Rpp40p, Rpp25p, and Rpp14p for RNase P, or pls 4 protein components namely Pop1p, Rpp29p, Rpp20p, and Rpp30p for RNase MRP, overview Homo sapiens

Synonyms

Synonyms Comment Organism
RNase MRP
-
 Homo sapiens
RNase MRP
-
 Saccharomyces cerevisiae
RNase P
-
 Cyanophora paradoxa
RNase P
-
 Bacteria
RNase P
-
 Bacillus subtilis
RNase P
-
 Homo sapiens
RNase P
-
 Saccharomyces cerevisiae
RNase P
-
 Spinacia oleracea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacteria
1.3
-
 tRNA precursor nuclear enzyme form Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae protein subunits Pop1p, Pop3p, Pop4p, Pop6p, Pop7p, Rpp1p, and Rpr2p 4.7 4.6
Saccharomyces cerevisiae protein subunit Pop8p
-
 4.9
Saccharomyces cerevisiae protein subunit Pop5p
-
 6.9