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Literature summary for 3.1.26.5 extracted from

  • Cech, T.R.; Bass, B.L.
    Biological catalysis by RNA (1986), Annu. Rev. Biochem., 55, 599-629.
    View publication on PubMed
Search for more literature for 3.1.26.5

General Stability

General Stability Organism
Ca2+, Sr2+ and Mg2+ maintain the structure of the enzyme in solution Escherichia coli
spermidine and spermine maintain the structure of the enzyme in solution Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+ strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA Bacillus subtilis
Mg2+ strict requirement for a divalent cation, Mg2+ or Mn2+. Hexacoordinated Mg2+ binds to the catalytic site on M1 RNA Escherichia coli
Mn2+ strict requirement for a divalent cation, Mg2+ or Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+ strict requirement for a divalent cation, Mg2+ or Mn2+ Bacillus subtilis
Mn2+ strict requirement for a divalent cation, Mg2+ or Mn2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pre-tRNA + H2O Salmonella enterica subsp. enterica serovar Typhimurium the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA + H2O Bacillus subtilis the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA + H2O Escherichia coli the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules tRNA + 5'-oligoribonucleotide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
ribonucleoprotein the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change Salmonella enterica subsp. enterica serovar Typhimurium
ribonucleoprotein the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change Bacillus subtilis
ribonucleoprotein the active site resides within the RNA moiety. The protein has a role in electrostatic shielding and aids in an essential conformational change Escherichia coli
ribonucleoprotein the enzyme requires an RNA as well as a protein subunit for its in vivo activity Salmonella enterica subsp. enterica serovar Typhimurium
ribonucleoprotein the enzyme requires an RNA as well as a protein subunit for its in vivo activity Bacillus subtilis
ribonucleoprotein the enzyme requires an RNA as well as a protein subunit for its in vivo activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pre-tRNA + H2O the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules Salmonella enterica subsp. enterica serovar Typhimurium tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA + H2O the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules Bacillus subtilis tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA + H2O the enzyme is responsible for the cleavage of sequences from the 5' ends of precursors of tRNAs to produce the mature 5' terminus of the tRNA molecules Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA precursor + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA precursor + H2O
-
 Bacillus subtilis tRNA + 5'-oligoribonucleotide
-
 ?
pre-tRNA precursor + H2O
-
 Escherichia coli tRNA + 5'-oligoribonucleotide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 9.5
-
 Escherichia coli