Literature summary for 3.1.26.4 extracted from

Lim, S.B.; Cha, S.H.; Jegal, S.; Jun, H.; Park, S.H.; Jeon, B.Y.; Pak, J.H.; Bakh, Y.Y.; Kim, T.S.; Lee, H.W.
Characterization of Echinostoma cinetorchis endoribonuclease, RNase H (2017), Korean J. Parasitol., 55, 451-455 .

Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment)	Organism
EcRNH, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of soluble GST-tagged enzyme in Escherichia coli strain BL21(DE3) plysS, subcloning in Escherichia coli strain DH5alpha	Echinostoma cinetorchis

Organism

Organism	UniProt	Comment	Textmining
Echinostoma cinetorchis	A0A3S8Z5R6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant soluble GST-tagged enzyme from Escherichia coli strain BL21(DE3) plysS by glutathione affinity chromatography, tag cleavage by thrombin, and gel filtration	Echinostoma cinetorchis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	recombinant EcRNH produced as a soluble form in Escherichia coli shows enzymatic activity to cleave the 3'-O-P bond of RNA in a DNA-RNA duplex, producing 3'-hydroxyl and 5'-phosphate	Echinostoma cinetorchis	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 34000, detagged recombinant enzyme, SDS-PAGE	Echinostoma cinetorchis

Synonyms

Synonyms	Comment	Organism
EcRNH	-	Echinostoma cinetorchis
RNase H	-	Echinostoma cinetorchis

General Information

General Information	Comment	Organism
additional information	enzyme EcRNH has an active site centered on a putative DDEED motif inxadstead of DEDD conserved in other species	Echinostoma cinetorchis

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Release 2023.1 (January 2023)