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Literature summary for 3.1.26.4 extracted from

  • Burkhart, B.W.; Cubonova, L.; Heider, M.R.; Kelman, Z.; Reeve, J.N.; Santangelo, T.J.
    The GAN exonuclease or the Flap endonuclease Fen1 and RNase HII are necessary for viability of Thermococcus kodakarensis (2017), J. Bacteriol., 199, e00141-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Protein Variants

Protein Variants Comment Organism
additional information construction of a gene TK0805 deletion strain lacking RNase HII activity. The mutant strain shows reduced growth compared to wild-type Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis O74035
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-

Synonyms

Synonyms Comment Organism
ribonuclease HII
-
 Thermococcus kodakarensis
RNase HII
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 Thermococcus kodakarensis
RnhB
-
 Thermococcus kodakarensis
TK0805
-
 Thermococcus kodakarensis

General Information

General Information Comment Organism
malfunction strains with both flap endonuclease (Fen1) and RNase HII deleted grow well. GINS-associated nuclease, GAN, activity is therefore sufficient for viability in the absence of both RNase HII and Fen1, but it is not possible to construct a strain with both RNase HII and GAN deleted. Fen1 alone is therefore insufficient for viability in the absence of both RNase HII and GAN. Deletion of both Fen1 and GAN or of both RNase HII and GAN is lethal Thermococcus kodakarensis
physiological function RNase HII is necessary for viability of Thermococcus kodakarensis. RNase HII is proposed to participate in primer processing during Okazaki fragment maturation. In Thermococcus kodakarensis, either flap endonuclease (Fen1) or GINS-associated nuclease (GAN) activity is sufficient for viability. GAN can support growth in the absence of both Fen1 and RNase HII, but Fen1 and RNase HII are required for viability in the absence of GAN. Individually, Fen1, GAN, and RNase HII are not essential for viability Thermococcus kodakarensis