Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)[pLysS] cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H264A | the mutation causes an about 100fold decrease in kcat under multiple-turnover conditions, but does not alter the Km value. The H264A mutant is not rescued by increasing the Mg2+ concentration to 80 mM | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ is inhibitory at concentrations above 10 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000097 | - |
RNA-DNA heteroduplex | wild type enzyme, at pH 7.5 and 30°C | Homo sapiens | |
0.000106 | - |
RNA-DNA heteroduplex | mutant enzyme H264A, at pH 7.5 and 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM Co2+ supports activity, with only minor inhibition observed at higher concentrations | Homo sapiens | |
K+ | optimum KCl concentration of 100-150 mM | Homo sapiens | |
Mg2+ | Mg2+ best supports the enzyme, with an optimal concentration of 10 mM | Homo sapiens | |
Mn2+ | 5 mM Mn2+ supports activity, with only minor inhibition observed at higher concentrations | Homo sapiens | |
additional information | not stimulated by Ca2+ | Homo sapiens | |
Ni2+ | exhibits only modest activity as cofactor | Homo sapiens | |
Zn2+ | exhibits only modest activity as cofactor | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA heteroduplex + H2O | Homo sapiens | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA heteroduplex + H2O | - |
Homo sapiens | ribonucleotide 5'-phosphomonoester + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
RNA-DNA heteroduplex | mutant enzyme H264A, at pH 7.5 and 30°C | Homo sapiens | |
3.5 | - |
RNA-DNA heteroduplex | wild type enzyme, at pH 7.5 and 30°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in Hepes buffer | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | more than 60% activity at pH 6.5, about 70% activity at pH 7.0, maximum activity at pH 7.5, more than 80% activity between pH 8.0 and 11.0 | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
RNA-DNA heteroduplex | mutant enzyme H264A, at pH 7.5 and 30°C | Homo sapiens | |
36000 | - |
RNA-DNA heteroduplex | wild type enzyme, at pH 7.5 and 30°C | Homo sapiens |