Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.4 extracted from

  • Ohtani, N.; Yanagawa, H.; Tomita, M.; Itaya, M.
    Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7 (2004), Nucleic Acids Res., 32, 5809-5819.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Protein Variants

Protein Variants Comment Organism
D125N mutation results in loss of dsRNase activity but not RNase H activity Sulfurisphaera tokodaii
D76N no RNase H activity is detected Sulfurisphaera tokodaii
D7N enzyme loses RNase H activity and dsRNase activity in the presence of MnCl2 Sulfurisphaera tokodaii
E52Q no RNase H activity is detected Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions. The enzyme cleaves an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii
Mg2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions Sulfurisphaera tokodaii
Mn2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions. The enzyme cleaves an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii
Ni2+ the enzyme cleaves an RNA strand of the 12-bp RNA/DNA hybrid at multiple sites only in the presence of Mn2+, Mg2+, Co2+ or Ni2+, but not in the presence of Cu2+, Ca2+ or Zn2+, or in the absence of divalent metal ions Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 gel filtration Sulfurisphaera tokodaii
16812
-
 1 * 16812, calculated from sequence Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii F9VN79
-
-
Sulfurisphaera tokodaii 7 F9VN79
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O the enzyme cleaves the RNA strand of an RNA/DNA hybrid or an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii ?
-
 ?
RNA + H2O the enzyme cleaves the RNA strand of an RNA/DNA hybrid or an RNA/RNA duplex in the presence of Mn2+ or Co2+ Sulfurisphaera tokodaii 7 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 16812, calculated from sequence Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
protein ST0753
-
 Sulfurisphaera tokodaii
RNase HI
-
 Sulfurisphaera tokodaii
type 1 RNase H
-
 Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 the standard cleavage reaction is performed at a pH of 8.5 in the presence of 1 mM MgCl2 or MnCl2, because at a higher pH range, the solubility of divalent metal ions decreases and RNA/DNA substrates might become destabilized Sulfurisphaera tokodaii
10
-
 the cleavage activity increases exponentially as the pH increases from 6 to 10 Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 the cleavage activity increases exponentially as the pH increases from 6 to 10 Sulfurisphaera tokodaii

pI Value

Organism Comment pI Value Maximum pI Value
Sulfurisphaera tokodaii calculated from sequence
-
 9.5