Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.26.4 extracted from

  • Watkins, H.A.; Baker, E.N.
    Structural and functional characterisation of an RNase HI domain from the bifunctional protein Rv2228c from Mycobacterium tuberculosis (2010), J. Bacteriol., 192, 2878-2886.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
fusion protein of maltose binding protein and the N-terminal RNase H domain, to 2.2.5 A resolution. Protein is monomeric in solution but associates in the crystal to form a dimer Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
additional information fusion protein of maltose binding protein and the N-terminal RNase H domain shows RNase H activity with a hybrid RNA/DNA substrate as well as double-stranded RNase activity. The full-length protein has additional CobC activity Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WLH5 bifunctional protein, the N-terminal domain is homologous with prokaryotic and eukaryotic RNase H domains and the C-terminal domain with alpha-ribazole phosphatase CobC
-
Mycobacterium tuberculosis H37Rv P9WLH5 bifunctional protein, the N-terminal domain is homologous with prokaryotic and eukaryotic RNase H domains and the C-terminal domain with alpha-ribazole phosphatase CobC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA duplex + H2O substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain Mycobacterium tuberculosis ?
-
 ?
RNA-DNA duplex + H2O substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain Mycobacterium tuberculosis H37Rv ?
-
 ?
RNA-RNA duplex + H2O substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain Mycobacterium tuberculosis ?
-
 ?
RNA-RNA duplex + H2O substrate both for full-lentgh enzyme and isolated RNase H N-terminal RNase H domain Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

Synonyms Comment Organism
Rv2228c
-
 Mycobacterium tuberculosis