Cloned (Comment) | Organism |
---|---|
expression of His-tagged RNase H1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | multiple-turnover kinetics with heteroduplexes | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | Homo sapiens | - |
DNA + 5'-phosphonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged RNase H1 from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography followed by reverse phase chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-2'-methoxyethoxy-antisense RNA hybrid + H2O | 2'-methoxyethoxy nucleotides, positioned at the 3' and 5' poles, into the antisense oligodeoxyribonucleotide of the heteroduplex to alter the helical geometry of the substrate | Homo sapiens | ? | - |
? | |
DNA-RNA hybrid + H2O | - |
Homo sapiens | DNA + 5'-phosphonucleotides | - |
? | |
additional information | selective substrate recognition by RNase H1 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |