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Literature summary for 3.1.26.4 extracted from
- Controlling RNA digestion by RNase H with a light-activated DNA hairpin (2006), Angew. Chem., 45, 3523-3526.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | mRNA/antisense oligodeoxynucleotide duplex formation activates RNase H-mediated hydrolysis of mRNA | Homo sapiens |
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | the enzyme can be useful in regulation of RNA degradation in applications of technologies involving mRNA, method development, overview | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | thermodynamics, overview | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA hybrid + H2O | Homo sapiens | strategy for regulating RNA digestion by RNase H by using a light-activated DNA hairpin, overview | DNA + 5'-phosphonucleotides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA-RNA hybrid + H2O | - |
Homo sapiens | DNA + 5'-phosphonucleotides | - |
? | |
| DNA-RNA hybrid + H2O | strategy for regulating RNA digestion by RNase H by using a light-activated DNA hairpin, overview | Homo sapiens | DNA + 5'-phosphonucleotides | - |
? | |
| additional information | design of a light-activated DNA hairpin to control the RNase H-mediated hydrolysis of mRNA, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
melting temperatures for duplexes of antisense oligodeoxynucleotide with RNA-15 and RNA-20 were 63°C and 70°C, respectively | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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