Any feedback?
Literature summary for 3.1.26.4 extracted from
- Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form (2000), J. Biochem., 127, 895-899.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rnhB, overexpression of the enzyme in an insoluble form in different strains of Escherichia coli, overexpression of soluble enzyme with a self-cleavable intein-tag | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates by 50% at 50 mM, inhibits by 90% at 200 mM | Escherichia coli |  |
| NaCl | activates by 50% at 50 mM, inhibits by 90% at 200 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00026 | - |
RNA*DNA hybrid | pH 8.5, 30°C, recombinant soluble non-tagged enzyme | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates at 50 mM, inhibits at 200 mM | Escherichia coli | |
| Mg2+ | absolutely dependent on for activity, can be substituted by Mn2+ | Escherichia coli | |
| Mn2+ | absolutely dependent on for activity, can be substituted by Mg2+ | Escherichia coli | |
| additional information | no activity in absence of Mg2+ or Mn2+, and in presence of 10 mM of Ba2+, Ca2+, Co2+, Zn2+, Cu2+, Fe2+, or Sr2+ | Escherichia coli | |
| NaCl | activates at 50 mM, inhibits at 200 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
recombinant soluble non-tagged enzyme, gel filtration | Escherichia coli |
| 23000 | - |
1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
type II enzyme, gene rnhB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant insoluble enzyme in a urea-denatured form, recombinant soluble intein-tagged enzyme | Escherichia coli |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding from purified urea-denatured form with low yield | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the activity of the recombinant soluble enzyme is slightly higher than that of the recombinant refolded enzyme | Escherichia coli |
| 0.75 | - |
recombinant soluble nontagged enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA*DNA hybrid + H2O | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 23000, recombinant soluble non-tagged enzyme, SDS-PAGE | Escherichia coli |
| More | helical content of the enzyme structure is 23% | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease HII | - |
Escherichia coli |
| RNase H | - |
Escherichia coli |
| RNase HII | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.8 | - |
above | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.8 | enzyme is inactive below pH 7.0, activity increases with increasing pH | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
