Cloned (Comment) | Organism |
---|---|
expression of wild-type and N-terminally truncated mutant enzymes as GST-fusion proteins in Escherichia coli temperature sensitive mutant strain MIC3001 | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several N-terminally truncated enzyme forms for investigation of structure-function relationship | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | can substitute for Mg2+, activates N-terminally truncated mutant RNHIDELTA47 and inhibits the full length enzyme dependent on the presence of the N-terminal 47 amino acids | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | enzyme contains a nuclear-targeting domain | Trypanosoma brucei | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, can substitute for Mg2+, activates the full length enzyme dependent on the N-terminal 47 amino acids | Trypanosoma brucei | |
Mn2+ | can substitute for Mg2+, activates N-terminally truncated mutant RNHIDELTA47 and inhibits the full length enzyme dependent on the presence of the N-terminal 47 amino acids | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
type I enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli | Trypanosoma brucei |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Endonucleolytic cleavage to a 5'-phosphomonoester | structure-function relationship, a 42 amino acid noncanonical spacer domain is essential for enzyme function, the nuclear localization domain is not required for function but for RNA binding, it modulates the enzyme manganese-dependent activity | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA*DNA hybrid + H2O | hydrolyses the RNA strang of the RNA*DNA heteroduplex | Trypanosoma brucei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | domain organization, the nuclear targeting, which also binds RNA, and the catalytic domains are localized distinctly to the N-terminal and C-terminal parts bound by a spacer domain | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease H | - |
Trypanosoma brucei |
RNase HI | - |
Trypanosoma brucei |