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Literature summary for 3.1.26.4 extracted from

  • Eder, P.S.; Walder, J.A.
    Ribonuclease H from K562 human erythroleukemia cells. Purification, characterization, and substrate specificity (1991), J. Biol. Chem., 266, 6472-6479.
    View publication on PubMed
Search for more literature for 3.1.26.4

Inhibitors

Inhibitors Comment Organism Structure
KCl half-maximal inhibition at 150 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent metal ion required. Maximal activity is obtained with 10 mM Mg2+, 5 mM Co2+ or 0.5 mM Mn2+ Homo sapiens
KCl activity increases with concentrations up to 50 mM Homo sapiens
Mg2+ divalent metal ion required. Maximal activity is obtained with 10 mM Mg2+, 5 mM Co2+ or 0.5 mM Mn2+ Homo sapiens
Mn2+ divalent metal ion required. Maximal activity is obtained with 10 mM Mg2+, 5 mM Co2+ or 0.5 mM Mn2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 sucrose density gradient centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA-DNA hybrid + H2O Homo sapiens the enzyme may play a role in ribonucleotide excision from genomic DNA during replication ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
RNase H1 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
K-562 cell K562 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme lacks double-stranded and single-stranded RNase and DNase activities. No hydrolysis of the DNA moiety of the RNA/DNA heteroduplex Homo sapiens ?
-
 ?
RNA-DNA hybrid + H2O the enzyme can hydrolyze a DNA*RNA*DNA/DNA heteroduplex that contains a single ribonucleotide Homo sapiens ribonucleotide 5'-phosphomonoester
-
 ?
RNA-DNA hybrid + H2O poly(rUdA) Homo sapiens ribonucleotide 5'-phosphomonoester
-
 ?
RNA-DNA hybrid + H2O poly(rAdT) Homo sapiens ribonucleotide 5'-phosphomonoester
-
 ?
RNA-DNA hybrid + H2O the enzyme may play a role in ribonucleotide excision from genomic DNA during replication Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
?
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 in presence of 10 mM Mg2+ Homo sapiens