Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | 20%, 50% loss of activity | Thermus thermophilus | |
SDS | 0.002%, 50% loss of activity | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermus thermophilus | |
additional information | - |
additional information | - |
Escherichia coli | |
0.0005 | - |
M13 DNA/RNA | at 37°C | Thermus thermophilus | |
0.0011 | - |
M13 DNA/RNA | at 70°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | required | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
M13 DNA/RNA + H2O | - |
Thermus thermophilus | ? | - |
? | |
M13 DNA/RNA + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
RNA-DNA hybrid + H2O | - |
Escherichia coli | ribonucleotide 5'-phosphomonoester | - |
? | |
RNA-DNA hybrid + H2O | M13 DNA/RNA hybrid | Thermus thermophilus | ribonucleotide 5'-phosphomonoester | - |
? | |
RNA-DNA hybrid + H2O | M13 DNA/RNA hybrid | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ribonucleotide 5'-phosphomonoester | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 21000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48.2 | - |
melting temperature in presence of 1.2 M guanidinium hydrochloride | Escherichia coli |
55 | - |
10 min, 60% loss of activity | Escherichia coli |
82.1 | - |
melting temperature in presence of 1.2 M guanidinium hydrochloride | Thermus thermophilus |
90 | - |
10 min, stable | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermus thermophilus | |
additional information | - |
additional information | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | cleavage of M13 DNA/RNA hybrid | Thermus thermophilus |