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Literature summary for 3.1.26.4 extracted from

  • Cathala, G.; Rech, J.; Huet, J.; Jeanteur, P.
    Isolation and characterization of two types of ribonucleases H in Krebs II ascites cells (1979), J. Biol. Chem., 254, 7353-7361.
    View publication on PubMed
Search for more literature for 3.1.26.4

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol enzyme form H2 is inactive in absence of mercaptoethanol. No effect on enzyme form H1 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Dextran
-
 Mus musculus
EDTA
-
 Mus musculus
Ethidium bromide
-
 Mus musculus
KCl activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM Mus musculus
NaCl activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM Mus musculus
NEM enzyme form H2. No effect on enzyme form H1 Mus musculus
NH4Cl activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM Mus musculus
Nucleic acids enzyme form H1 is more susceptible to inhibition than enzyme form H2 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mus musculus
0.0005
-
 poly(rAdT) lower than 0.0005 mM Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. KCl is more efficient than NaCl Mus musculus
Mg2+ enzyme form H2: requirement for divalent metal ion can be satisfied only by Mg2+. Enzyme form H1: requirement for a divalent metal ion can be satisfied by Mg2+ or with a stronger preference with Mn2+ Mus musculus
Mn2+ enzyme form H1: requirement for a divalent metal ion can be satisfied by Mg2+ or with a stronger preference with Mn2+ Mus musculus
NaCl enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. KCl or NH4Cl is more efficient than NaCl Mus musculus
NH4Cl enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. NH4Cl is more efficient than NaCl Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, enzyme form H2, SDS-PAGE Mus musculus
36000 40000 enzyme form H2, gel filtration Mus musculus
105000 110000 enzyme form H1, gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, two types of enzyme: H1 and H2 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Krebs ascites cell
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(rAdT) + H2O
-
 Mus musculus ?
-
 ?
RNA-DNA hybrid + H2O poly(rAdT) Mus musculus ribonucleotide 5'-phosphomonoester
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, enzyme form H2, SDS-PAGE Mus musculus