Literature summary for 3.1.26.4 extracted from

Itaya, M.
Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene (1990), Proc. Natl. Acad. Sci. USA, 87, 8587-8591.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA-RNA hybrid duplex + H2O	Escherichia coli	-	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Renatured (Commentary)

Renatured (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000038	-	-	Escherichia coli

Storage Stability

Storage Stability	Organism
-20°C, 0.025 mg enzyme per ml, in 0.1 M Tris-HCl, pH 7.5, 0.01 MgCl2, 20 mM urea, 1 mM DTT, 0.1% Triton X-100, 50% v/v glycerol, stable for several weeks	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA-RNA hybrid duplex + H2O	-	Escherichia coli	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?
DNA-RNA hybrid duplex + H2O	preference for RNA-DNA hybrid but low activity towards ss and ds RNA and DNA	Escherichia coli	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?
DNA-RNA hybrid duplex + H2O	most active substrate: (rA)n-(dT)n	Escherichia coli	oligonucleotides terminated with 5'-phosphate and 3'-hydroxyl moiety	-	?

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Release 2023.1 (January 2023)