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Literature summary for 3.1.26.3 extracted from

  • Hotto, A.M.; Castandet, B.; Gilet, L.; Higdon, A.; Condon, C.; Stern, D.B.
    Arabidopsis chloroplast mini-ribonuclease III participates in rRNA maturation and intron recycling (2015), Plant Cell, 27, 724-740 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
gene At1g55140, sequence comparison and phylogenetic analysis Arabidopsis thaliana
gene At3g13740, sequence comparison and phylogenetic analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of null mutants for both genes RNC3 and RNC4 from the T-DNA insertion collection, termed rnc3-1 and rnc4-1, respectively, and of a double knockout mutant rnc3/4. Phenotypes, overview. rRNA deficiencies observed in rnc3/4 can be complemented by an RNC transgene Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q6NQK8
-
-
Arabidopsis thaliana Q94F12
-
-

Synonyms

Synonyms Comment Organism
At1g55140 locus name Arabidopsis thaliana
At3g13740 locus name Arabidopsis thaliana
RNase III
-
 Arabidopsis thaliana
RNC3
-
 Arabidopsis thaliana
RNC4
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
evolution the two Arabidopsis thaliana chloroplast Mini-RNase III-like enzymes share 75% amino acid sequence identity Arabidopsis thaliana
malfunction whereas rnc3 and rnc4 null mutants have no visible phenotype, rnc3/rnc4 (rnc3/4) double mutants are slightly smaller and chlorotic compared with the wild-type. Imprecise maturation of 23S rRNA is observed in the rnc3/4 double mutant, suggesting that exoribonucleases generate staggered ends in the absence of specific Mini-III-catalyzed cleavages. A similar phenotype is found at the 3' end of the 16S rRNA, and the primary 4.5S rRNA transcript contained 3' extensions, suggesting that Mini-III catalyzes several processing events of the polycistronic rRNA precursor. The rnc3/4 mutant shows overaccumulation of a noncoding RNA complementary to the 4.5S-5S rRNA intergenic region, and its presence correlates with that of the extended 4.5S rRNA precursor. Mature 23S rRNA 3' ends are unaffected by mini-RNase III deficiency Arabidopsis thaliana
metabolism the enzyme is involved in plastid rRNA maturation Arabidopsis thaliana
physiological function RNase III proteins recognize double-stranded RNA structures and catalyze endoribonucleolytic cleavages that often regulate gene expression. Arabidopsis thaliana chloroplast mini-ribonuclease III participates in rRNA maturation and intron recycling, overview. Key role of Mini-III in intron and noncoding RNA regulation Arabidopsis thaliana