Crystallization (Comment) | Organism |
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crystal structure of enzyme Rnt1p in complex with a G2 loop | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | the enzyme Rnt1p binds to RNA stems capped with an NGNN tetraloop, via specific interactions between a structural motif located at the end of the Rnt1p dsRNA-binding domain and the guanine nucleotide in the second position of the loop | ? | - |
? | |
additional information | Saccharomyces cerevisiae ATCC 204508 | the enzyme Rnt1p binds to RNA stems capped with an NGNN tetraloop, via specific interactions between a structural motif located at the end of the Rnt1p dsRNA-binding domain and the guanine nucleotide in the second position of the loop | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q02555 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | Q02555 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme Rnt1p binds to RNA stems capped with an NGNN tetraloop, via specific interactions between a structural motif located at the end of the Rnt1p dsRNA-binding domain and the guanine nucleotide in the second position of the loop | Saccharomyces cerevisiae | ? | - |
? | |
additional information | mode of substrate recognition by the enzyme, which has a unique RNA-binding motif, the enzyme interacts with the RNA stem upstream of the cleavage sites, structure-function analysis, detailed overview. Interaction between the N-terminal domain and RNA increases precision of cleavage site selection | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the enzyme Rnt1p binds to RNA stems capped with an NGNN tetraloop, via specific interactions between a structural motif located at the end of the Rnt1p dsRNA-binding domain and the guanine nucleotide in the second position of the loop | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
additional information | mode of substrate recognition by the enzyme, which has a unique RNA-binding motif, the enzyme interacts with the RNA stem upstream of the cleavage sites, structure-function analysis, detailed overview. Interaction between the N-terminal domain and RNA increases precision of cleavage site selection | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase III | - |
Saccharomyces cerevisiae |
Rnt1p | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the ribonuclease III (RNase III) family | Saccharomyces cerevisiae |
additional information | the dsRNA-binding domain and N-terminal domains of enzyme Rnt1p function as two rulers that measure the distance between the tetraloop and the cleavage site, mechanism, overview. Both rulers interact with the NGNN tetraloop: ruler 1 recognizes the Gua16 base, and ruler 2 secures the Gua16 recognition | Saccharomyces cerevisiae |