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Literature summary for 3.1.26.3 extracted from

  • Madina, B.R.; Kuppan, G.; Vashisht, A.A.; Liang, Y.H.; Downey, K.M.; Wohlschlegel, J.A.; Ji, X.; Sze, S.H.; Sacchettini, J.C.; Read, L.K.; Cruz-Reyes, J.
    Guide RNA biogenesis involves a novel RNase III family endoribonuclease in Trypanosoma brucei (2011), RNA, 17, 1821-1830.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Protein Variants

Protein Variants Comment Organism
D218A site-directed mutagenesis, inactive mutant Trypanosoma brucei
additional information inducible knockdown of mRPN1 in Trypanosoma brucei results in loss of gRNA and accumulation of precursor transcripts, consistent with a role of mRPN1 in processing Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Trypanosoma brucei 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei
additional information recombinant mRPN1 requires Mg2+ and a critical catalytic carboxylate Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57300
-
 purified recombinant His-tagged wild-type enzyme, gel filtration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei endonuclease mRPN1 directly binds with TbRGG2 and exhibits a nuclease-resistant association with two more proteins, 4160 and 8170, it might modulate gRNA utilization by editing complexes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information endonuclease mRPN1 directly binds with TbRGG2 and exhibits a nuclease-resistant association with two more proteins, 4160 and 8170, it might modulate gRNA utilization by editing complexes Trypanosoma brucei ?
-
 ?
additional information recombinant mRPN1 is a dimeric dsRNA-dependent endonuclease that generates 2-nucleotide 3' overhangs, cleavage specificity of mRPN1 is reminiscent of bacterial RNase III and thus is fundamentally distinct from editing endonucleases, overview Trypanosoma brucei ?
-
 ?

Subunits

Subunits Comment Organism
dimer homology model of mRPN1, overview Trypanosoma brucei

Synonyms

Synonyms Comment Organism
mitochondrial RNA precursor-processing endonuclease 1
-
 Trypanosoma brucei
mRPN1
-
 Trypanosoma brucei

General Information

General Information Comment Organism
evolution mRPN1 is a homologue of the REN endonucleases Trypanosoma brucei
malfunction inducible knockdown of mRPN1 in Trypanosoma brucei results in loss of gRNA and accumulation of precursor transcripts, consistent with a role of mRPN1 in processing Trypanosoma brucei
additional information four conserved catalytic side-chains E214, D218, D288, and E291 Trypanosoma brucei
physiological function recombinant mRPN1 is a dimeric dsRNA-dependent endonuclease that requires Mg2+, a critical catalytic carboxylate, and generates 2-nucleotide 3' overhangs. Minicircles in the mitochondrial genome encode hundreds of small guide RNAs, gRNAs, that partially anneal with unedited mRNAs and direct the extensive editing. Trypanosoma brucei gRNAs and mRNAs are transcribed as polycistronic precursors, which undergo processing preceding editing. The mitochondrial RNA precursor-processing endonuclease 1, mRPN1, is involved in gRNA biogenesis, overview Trypanosoma brucei