Cloned (Comment) | Organism |
---|---|
cloning of full-length cDNA of DICER1 protein from HeLa cell total RNA, and expression of N-terminally FLAG-tagged DICER1 protein in 293T cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | two-step cleavage of hairpin RNA with 5' overhangs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UPY3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | two-step cleavage of hairpin RNA with 5' overhangs | Homo sapiens | ? | - |
? | |
additional information | recombinant DICER protein processes a hairpin RNA with 5' overhangs in vitro and generates an intermediate duplex with a 29 nt-5' strand and a 23 nt-3' strand. Longer 5' overhangs with stable stem structures can reduce the efficiency or rate of substrate cleavage. In vitro two-step processing of the 5'-end labelled pre-mmu-mir-1982 RNA by recombinant DICER protein, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dicer | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | DICER is an RNase III family endoribonuclease that processes precursor microRNAs and long double-stranded RNAs, generating microRNA duplexes and short interfering RNA duplexes with 20-23 nucleotides in length | Homo sapiens |