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Literature summary for 3.1.26.3 extracted from

  • Nathania, L.; Nicholson, A.W.
    Thermotoga maritima ribonuclease III. Characterization of thermostable biochemical behavior and analysis of conserved base pairs that function as reactivity epitopes for the Thermotoga 23S rRNA precursor (2010), Biochemistry, 49, 7164-7178.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.26.3

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ less effective than Mg2+ Thermotoga maritima
Mg2+ activates optimally at 1 mM Thermotoga maritima
Mn2+ less effective than Mg2+ Thermotoga maritima
additional information the enzyme functions optimally at pH 8 and about 50-80 mM salt concentrations Thermotoga maritima
Ni2+ less effective than Mg2+ Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity with RNA duplex substrates, overview. The proximal box is a primary reactivity epitope for Tm-23S[hp] RNA. a CG or GC bp substitution at pb position 2 reduces the relative reactivity to 0.1 and 0.3, respectively, while a CG or GC substitution at pb position 4 provides a relative reactivity of 0.1 or 0.4, respectively. At pb position 3, only the AU bp substitution causes a significant drop in relative reactivity, while none of the bp substitutions at pb position 1 has a significant effect Thermotoga maritima ?
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Synonyms

Synonyms Comment Organism
ribonuclease III
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 Thermotoga maritima
RNase III
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 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 70 RNase III catalytic activity exhibits a broad optimal temperature range Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 95 activity range Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermotoga maritima

General Information

General Information Comment Organism
evolution Tm-RNase III cleaves an Ec-RNase III substrate with identical specificity and is inhibited by antideterminant bp that also inhibit Ec-RNase III, indicating the conservation, across a broad phylogenetic distance, of positive and negative determinants of reactivity of bacterial RNase III substrates Thermotoga maritima
physiological function cleavage of double-stranded RNA by ribonuclease III is a conserved early step in bacterial rRNA maturation, mechanism of dsRNA cleavage by RNase III, overview Thermotoga maritima