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Literature summary for 3.1.26.3 extracted from

  • Amarasinghe, A.K.; Calin-Jageman, I.; Harmouch, A.; Sun, W.; Nicholson, A.W.
    Escherichia coli ribonuclease III: affinity purification of hexahistidine-tagged enzyme and assays for substrate binding and cleavage (2001), Methods Enzymol., 342, 143-158.
    View publication on PubMed
Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment) Organism
gene rnc, mapping at 55 min on the bacterial chromosome, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Zn2+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 RNA 37°C Escherichia coli
0.34
-
 RNA recombinant His-tagged enzyme, pH 8.0, 35°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially substitute for Mg2+ Escherichia coli
Mg2+ dependent on Escherichia coli
Mn2+ can partially substitute for Mg2+ Escherichia coli
additional information no activity with Ca2+ and Sr2+ Escherichia coli
Ni2+ can partially substitute for Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25600
-
 x * 25600, amino acid sequence calculation, x * 29000, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli
29000
-
 x * 25600, amino acid sequence calculation, x * 29000, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsRNA + H2O Escherichia coli
-
 processed RNA
-
 ?
additional information Escherichia coli involved in the maturation of the ribosomal RNA precursor, and bacteriophage T7 mRNA precursors, enzyme participates in the degradation as well as maturation of diverse cellular, phage, and plasmid RNAs ?
-
 ?
pre-16S rRNA + H2O Escherichia coli
-
 mature 16S rRNA
-
 ?
pre-23S rRNA + H2O Escherichia coli
-
 mature 23S rRNA
-
 ?
pre-5S rRNA + H2O Escherichia coli
-
 mature 5S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme to over 90% purity Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
Endonucleolytic cleavage to a 5'-phosphomonoester determination of specific cleavage site, Glu117 is important for phosphodiester hydrolysis Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 gel mobility shift assay for substrate bindng determination Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsRNA + H2O
-
 Escherichia coli processed RNA
-
 ?
dsRNA + H2O specific for double-stranded RNA Escherichia coli processed RNA enzyme produces 12-15 base pair duplex products with 5'-phosphate, 3'-hydroxyl termini ?
additional information involved in the maturation of the ribosomal RNA precursor, and bacteriophage T7 mRNA precursors, enzyme participates in the degradation as well as maturation of diverse cellular, phage, and plasmid RNAs Escherichia coli ?
-
 ?
pre-16S rRNA + H2O
-
 Escherichia coli mature 16S rRNA
-
 ?
pre-23S rRNA + H2O
-
 Escherichia coli mature 23S rRNA
-
 ?
pre-5S rRNA + H2O
-
 Escherichia coli mature 5S rRNA
-
 ?
R1.1 RNA + H2O substrate is enzymatically synthesized based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, 1 cleavage site Escherichia coli 2 fragment of R1.1 RNA
-
 ?
RNA + H2O
-
 Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25600, amino acid sequence calculation, x * 29000, recombinant His-tagged enzyme, SDS-PAGE Escherichia coli
More the enzyme contains a dsRNA binding and a catalytic subdomain Escherichia coli

Synonyms

Synonyms Comment Organism
More enzyme belongs to the ribonuclease III superfamily Escherichia coli
RNase III
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 RNA recombinant His-tagged enzyme, pH 8.0, 35°C Escherichia coli
7.7
-
 RNA 37°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli