Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | stimualtion at 20 mM | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpression of the rnc gene in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | at concentrations higher than 0.5 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulation at 20 mM | Escherichia coli | |
Mg2+ | required | Escherichia coli | |
Mn2+ | stimulates | Escherichia coli | |
Mn2+ | optimal concentration at 0.15-0.3 mM | Escherichia coli | |
Na+ | stimualtion at 20 mM | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | Escherichia coli | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the overexpressed protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
poly(A)-poly(U) + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
ribosomal RNA + H2O | the small and stable 10Sa RNA is cleaved | Escherichia coli | smaller precursor rRNA | - |
? | |
ribosomal RNA + H2O | 7S RNA is cleaved | Escherichia coli | smaller precursor rRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
30 min, no loss af activity | Escherichia coli |
50 | - |
30 min, 50% loss af activity | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.4 | - |
Escherichia coli |