Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutamate | extends salt concentration range | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
F- | inhibits at 100 mM | Escherichia coli | |
Zn2+ | inhibits Mg2+ dependent reaction | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
RNA | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates | Escherichia coli | |
Mg2+ | stimulates | Escherichia coli | |
Mn2+ | stimulates | Escherichia coli | |
Zn2+ | inhibits Mg2+ dependent reaction | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | Escherichia coli | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the overexpressed protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | bacteriophage T7 RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
RNA + H2O | - |
Escherichia coli | ? | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Escherichia coli |