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Literature summary for 3.1.26.3 extracted from
- Ribonuclease III cleavage of a bacteriophage T7 processing signal. Divalent cation specificity, and specific anion effects (1993), Nucleic Acids Res., 21, 1919-1925.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutamate | extends salt concentration range | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | inhibits at 100 mM | Escherichia coli | |
| Zn2+ | inhibits Mg2+ dependent reaction | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00026 | - |
RNA | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Escherichia coli | |
| Mg2+ | stimulates | Escherichia coli | |
| Mn2+ | stimulates | Escherichia coli | |
| Zn2+ | inhibits Mg2+ dependent reaction | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded RNA + H2O | Escherichia coli | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the overexpressed protein | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded RNA + H2O | bacteriophage T7 RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
| RNA + H2O | - |
Escherichia coli | ? | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | - |
Escherichia coli |
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