Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | less than 1 mM | Escherichia coli | |
NH4+ | cleavage pattern depends on concentration | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required | Escherichia coli | |
Co2+ | - |
Escherichia coli | |
Cu2+ | - |
Escherichia coli | |
Double-stranded RNA | inhibits cleavage of single-stranded RNA | Escherichia coli | |
EDTA | greater than 10 mM | Escherichia coli | |
Fe3+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
K+ | - |
Escherichia coli | |
Mn2+ | - |
Escherichia coli | |
NaCl | 0.15-0.3 M cleaves at primary cleavage sites, inhibition at higher concentrations, at 5 mM additional secondary cleavage sites | Escherichia coli | |
NH4+ | concentration dependent cleavage pattern | Escherichia coli | |
poly(IC) | as double-stranded RNA | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits | Escherichia coli | |
Mg2+ | - |
Escherichia coli | |
Mn2+ | inhibits | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | 55000 | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | Escherichia coli | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | 5'-phosphooligonucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA | Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
single-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
tRNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | no activity at 0°C and 17.5°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
nonspecific cleavage of RNA after heating for 1 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | inactive below, RNA | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Escherichia coli |