Literature summary for 3.1.26.3 extracted from

Birenbaum, M.; Shen, V.; Nikolaev, N.; Schlessinger, D.
Site-specific processing of Escherichia coli preribosomal RNA and preribosomes by E. coli RNase III (1979), Methods Enzymol., 59, 824-837.

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
double-stranded RNA + H2O	Escherichia coli	cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA	5'-phosphooligonucleotides	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.4	-	-	Escherichia coli

Storage Stability

Storage Stability	Organism
-20°C, 25 mM Tris, pH 8, 8% (NH4)2SO4, 50% glycerol, several months, no loss of activity	Escherichia coli
-70°C, 1.3 M NH4Cl, 2 years, 40-50% loss of activity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
double-stranded RNA + H2O	-	Escherichia coli	5'-phosphooligonucleotides	-	?
double-stranded RNA + H2O	cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA	Escherichia coli	5'-phosphooligonucleotides	-	?
ribosomal RNA + H2O	-	Escherichia coli	smaller precursor rRNA	-	?

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Release 2023.1 (January 2023)