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Literature summary for 3.1.26.2 extracted from
- A ribonuclease specific for 2'-methylated ribonucleic acid (1967), J. Biol. Chem., 242, 2029-2034.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM | Synechococcus elongatus PCC 7942 = FACHB-805 |  |
| additional information | 1 mM Hg2+ has no effect | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Ni2+ | 1 mM | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Pb2+ | 1 mM | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| Zn2+ | 1 mM | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no hydrolysis of: deoxyribonucleic acid, normal yeast RNA | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | - |
? | |
| RNA, methylated in the 2'-hydroxy position of ribose + H2O | chemically 2'-O-methylated yeast RNA | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | the products are terminated in 5'-phosphates | ? | |
| RNA, methylated in the 2'-hydroxy position of ribose + H2O | naturally occuring 2'-O-methyl RNA isolated from rhapidosomes | Synechococcus elongatus PCC 7942 = FACHB-805 | ? | the products are terminated in 5'-phosphates | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2'-O-methyl RNase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, retains almost two-thirds of its initial activity | Synechococcus elongatus PCC 7942 = FACHB-805 |
| 95 | - |
5 min, complete inactivation | Synechococcus elongatus PCC 7942 = FACHB-805 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7.5 | pH 4.0: considerable activity at, pH 7.5: optimum | Synechococcus elongatus PCC 7942 = FACHB-805 |
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Release 2023.1 (January 2023)
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