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Literature summary for 3.1.26.13 extracted from
- Template-primer binding affinity and RNase H cleavage specificity contribute to the strand transfer efficiency of HIV-1 reverse transcriptase (2018), J. Biol. Chem., 293, 13351-13363 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D443N | RNase H inactivating mutation, the mutant is devoid of strand transfer activity | Human immunodeficiency virus type 1 group M subtype B |
| D443N | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| E478Q | RNase H inactivating mutation, the mutant is devoid of strand transfer activity | Human immunodeficiency virus type 1 group M subtype B |
| E478Q | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| F61A | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| K358R/A359G/S360A | the mutant shows highly increased strand transfer efficiency compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| K65R/E478Q | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| L92P | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| L92P/D443N | site-directed mutagenesis, the mutant shows decreased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| L92P/E478Q | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| additional information | the kinetics of wild-type and mutant enzymes are determined with the RNA/DNA template-primer R33B/20A. Rates of nontemplated nucleotide addition of wild-type and mutant HIV-1 RTs using blunt-ended DNA/DNA substrates, overview. Non-templated nucleotide addition and strand transfer are mechanistically independent | Human immunodeficiency virus type 1 group O subtype B |
| T355A/Q357M | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| T355A/Q357MK358R/A359G/S360A | the mutant shows highly increased strand transfer efficiency compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| V148I | site-directed mutagenesis, the mutant shows increased dissociation rate constant (koff) compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
| V148I/K358R/A359G/S360A | the mutant shows highly increased strand transfer efficiency compared to wild-type | Human immunodeficiency virus type 1 group O subtype B |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of wild-type and mutant enzymes are determined with the RNA/DNA template-primer R33B/20A | Human immunodeficiency virus type 1 group O subtype B | |
| additional information | - |
additional information | kinetics of wild-type enzyme are determined with the RNA/DNA template-primer R33B/20A | Human immunodeficiency virus type 1 group M subtype B |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Human immunodeficiency virus type 1 group M subtype B | P03366 | HIV-1 | - |
| Human immunodeficiency virus type 1 group M subtype B BH10 | P03366 | HIV-1 | - |
| Human immunodeficiency virus type 1 group O subtype B | - |
HIV-1 | - |
| Human immunodeficiency virus type 1 group O subtype B | - |
HIV-1ESP49 | - |
| Human immunodeficiency virus type 1 group O subtype B ESP49 | - |
HIV-1 | - |
| Human immunodeficiency virus type 1 group O subtype B ESP49 | - |
HIV-1ESP49 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | activities of wild-type and mutant enzymes are determined with the RNA/DNA template-primer R33B/20A, comparison of HIV-1 group O subtype B ESP49 and HIV-1 group M subtype B BH10 | Human immunodeficiency virus type 1 group O subtype B | ? | - |
? |  |
| additional information | activity of wild-type enzyme is determined with the RNA/DNA template-primer R33B/20A, comparison of HIV-1 group O subtype B ESP49 and HIV-1 group M subtype B BH10 | Human immunodeficiency virus type 1 group M subtype B | ? | - |
? | |
| additional information | activities of wild-type and mutant enzymes are determined with the RNA/DNA template-primer R33B/20A, comparison of HIV-1 group O subtype B ESP49 and HIV-1 group M subtype B BH10 | Human immunodeficiency virus type 1 group O subtype B ESP49 | ? | - |
? | |
| additional information | activity of wild-type enzyme is determined with the RNA/DNA template-primer R33B/20A, comparison of HIV-1 group O subtype B ESP49 and HIV-1 group M subtype B BH10 | Human immunodeficiency virus type 1 group M subtype B BH10 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H | - |
Human immunodeficiency virus type 1 group M subtype B |
| RNase H | - |
Human immunodeficiency virus type 1 group O subtype B |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the substitution of Pro for Leu-92 renders an HIV-1 reverse transcriptase without strand transfer activity but retaining wild-type DNA polymerase and RNase H activities. Introduction of L92P produces a 3-4fold increase in the dissociation equilibrium constant (Kd) for DNA/DNA template-primers, suggesting that the strand transfer defect is a consequence of impaired nucleic acid binding | Human immunodeficiency virus type 1 group O subtype B |
| additional information | nucleotide analogues can enhance strand transfer by slowing DNA polymerization kinetics. Contribution of dissociation rate constants (koff) to the strand transfer efficiency of HIV-1 RTs, overview | Human immunodeficiency virus type 1 group M subtype B |
| additional information | nucleotide analogues can enhance strand transfer by slowing DNA polymerization kinetics. Contribution of dissociation rate constants (koff) to the strand transfer efficiency of HIV-1 RTs, overview | Human immunodeficiency virus type 1 group O subtype B |
| physiological function | template-primer binding affinity and RNase H cleavage specificity contribute to the strand transfer efficiency of HIV-1 reverse transcriptase | Human immunodeficiency virus type 1 group M subtype B |
| physiological function | template-primer binding affinity and RNase H cleavage specificity contribute to the strand transfer efficiency of HIV-1 reverse transcriptase | Human immunodeficiency virus type 1 group O subtype B |
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